Elevated tumor necrosis factor-alpha expression in periapical lesions infected by Epstein-Barr virus
| Autor: | József Kónya, Levente Szakács, Richard Mogyorósi, Eszter Csoma, Anita Szalmás, Ildikó Márton, Beáta Mészáros, Katinka Hernádi, György Veress, Eszter Gyöngyösi, István Varga, Levente Czompa |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Human cytomegalovirus
Adult Pathology medicine.medical_specialty Epstein-Barr Virus Infections Herpesvirus 4 Human viruses Herpesvirus 6 Human Cytomegalovirus Biology Klinikai orvostudományok medicine.disease_cause Real-Time Polymerase Chain Reaction Virus Statistics Nonparametric Lesion Transforming Growth Factor beta medicine Humans General Dentistry Periodontitis Chi-Square Distribution Tumor Necrosis Factor-alpha Orvostudományok Middle Aged medicine.disease biology.organism_classification Epstein–Barr virus Real-time polymerase chain reaction Case-Control Studies Immunology DNA Viral Herpesvirus 8 Human Human herpesvirus 6 medicine.symptom Nested polymerase chain reaction Periapical Periodontitis |
| Zdroj: | Journal of endodontics. 39(4) |
| ISSN: | 1878-3554 |
| Popis: | In apical periodontitis, there is an intense inflammatory response to endodontopathogenic bacteria, an essential component of the pathogenic microbiota. The inflammation can be aggravated by herpesviruses acting as nonessential pathogens in periapical lesions. This study aimed to determine the levels of tumor necrosis factor-alpha (TNF-α) and transforming growth factor-beta (TGF-β) in periapical lesions in relation to local occurrence of Epstein-Barr virus (EBV), human cytomegalovirus (HCMV), human herpesvirus 6 (HHV-6), and human herpesvirus 8 (HHV-8).Fifty-eight samples with apical periodontitis and 20 clinically healthy gingival control tissues were collected. Viral DNA was determined with nested polymerase chain reaction, and cytokine mRNA expression was detected with real-time polymerase chain reaction assays.Periapical lesions harbored EBV (75.9%) and HHV-6 (22.4%) at significantly higher frequencies compared with controls (P.000001 and P.05, respectively), whereas HCMV (12%) and HHV-8 (0%) occurred rarely. The median TNF-α expression was 13 times higher (P.001) and TGF-β expression was 5 times higher in periapical lesions than in controls (P.001). TNF-α expression was significantly higher in EBV-positive lesions than in EBV-negative lesions (P = .032). Presence of symptoms, lesion size, and infection by HCMV or HHV-6 had no significant association with either TNF-α or TGF-β expression.The herpesviral component of the endodontic microbiota did not correlate with TGF-β expression, whereas EBV infection was associated with a median 1.5 times further elevation of the high TNF-α expression characteristic for periapical lesions. |
| Databáze: | OpenAIRE |
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