The impact of ovarian stimulation protocol on oocyte quality, subsequent in vitro embryo development, and pregnancy after transfer to recipients in Eld's deer (Rucervus eldii thamin)

Autor: Daraka Tongthainan, D.K. Berg, Chainarong Punkong, Boripat Siriaroonrat, Umaporn Maikeaw, Wanlaya Tipkantha, Ampika Thongphakdee, Nudthakamol Kajornklin, Sakhon Noimoon, Pierre Comizzoli, Nikorn Thongtip, Theerawat Tharasanit, Sumate Kamolnorranath
Rok vydání: 2016
Předmět:
endocrine system
medicine.medical_specialty
medicine.medical_treatment
In Vitro Oocyte Maturation Techniques
Embryonic Development
Fertilization in Vitro
Biology
Andrology
03 medical and health sciences
Follicle-stimulating hormone
0302 clinical medicine
Food Animals
Ovulation Induction
Internal medicine
medicine
Animals
Blastocyst
Small Animals
030219 obstetrics & reproductive medicine
Equine
Deer
0402 animal and dairy science
04 agricultural and veterinary sciences
Oocyte
Embryo Transfer
040201 dairy & animal science
Buserelin
Embryo transfer
Endocrinology
medicine.anatomical_structure
embryonic structures
Oocytes
Gestation
Animal Science and Zoology
Ovulation induction
Female
Follicle Stimulating Hormone
Estrus Synchronization
hormones
hormone substitutes
and hormone antagonists
medicine.drug
Zdroj: Theriogenology. 91
ISSN: 1879-3231
Popis: Propagating genetically valuable individuals through oocyte collection, in vitro fertilization (IVF) and embryo transfer is critical to maintain sustainable populations of the endangered Eld's deer. The objectives of this study were to assess the impact of exogenous FSH injections on (1) the number and in vitro competence of oocytes collected and (2) the developmental potential of resulting IVF embryos after transfer into recipients during the breeding season (February-April). In a pilot experiment, estrus synchronization was conducted in three surplus females (using intravaginal progesterone-releasing devices, CIDRG for 14 days and injections of buserelin (a GnRH agonist). Five days after CIDR removal, ovaries were excised, minced and a total of 133 oocytes were recovered. Following in vitro maturation (IVM) and IVF, 63% of the oocytes formed embryos but only 5% reached the blastocyst stage. In a subsequent study, follicle numbers and diameters were compared between synchronized does stimulated with 0 or 80 mg FSH (-FSH and +FSH; n = 8 does in each group) and oocytes collected either by laparoscopic ovum pick-up or ovariectomy. FSH stimulation increased the main follicular diameter from 2-3 mm to 4-5 mm (P 0.05) but not the oocyte number (∼20/donor) or the percentage of good quality oocytes (57%) regardless of the treatment. FSH stimulation did not either affect the percentage of cleaved embryos after IVF (25-35%; P 0.05). Lastly, embryos at the 2-to 8-cell stage (from either + FSH or -FSH groups) were transferred into the oviducts of 11 synchronized recipients. With the +FSH embryos, three pregnancies failed between 90 and 120 days of gestation and two fawns that were born preterm (Days 215 and 224 of gestation) died at birth. In the -FSH group one healthy female fawn was born on Day 234 of gestation. This is the first report of a successful in vitro embryo production and subsequent birth of a live Eld's deer fawn. Further investigations are required to improve IVM/IVF success and the developmental potential of the embryos.
Databáze: OpenAIRE
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