miR-206 is required for changes in cell adhesion that drive muscle cell morphogenesis in Xenopus laevis
| Autor: | Parag Saraf, Melissa Adams, Trista Rosing, Ceazar Nave, Julio Ramirez, Carmen R. Domingo, Daniel Saw, Rebecca Blandino, Hernando Martínez Vergara, Coohleen Coombes, Gabriel Piexoto |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Notochord Morphogenesis Xenopus Xenopus Proteins Muscle Development Xenopus laevis 03 medical and health sciences 0302 clinical medicine Sequence Homology Nucleic Acid Somitogenesis Cell Adhesion medicine Animals Myocyte Dystroglycans Cell adhesion Cell Shape Molecular Biology Actin Muscle Cells biology Muscles Gene Expression Regulation Developmental Skeletal muscle Cell Biology biology.organism_classification Actins Cell biology MicroRNAs 030104 developmental biology medicine.anatomical_structure Somites 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental Biology. 438:94-110 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2018.03.021 |
| Popis: | MicroRNAs (miRNAs) are highly conserved small non-coding RNA molecules that post-transcriptionally regulate gene expression in multicellular organisms. Within the set of muscle-specific miRNAs, miR-206 expression is largely restricted to skeletal muscle and is found exclusively within the bony fish lineage. Although many studies have implicated miR-206 in muscle maintenance and disease, its role in skeletal muscle development remains largely unknown. Here, we examine the role of miR-206 during Xenopus laevis somitogenesis. In Xenopus laevis, miR-206 expression coincides with the onset of somitogenesis. We show that both knockdown and over-expression of miR-206 result in abnormal somite formation affecting muscle cell rotation, attachment, and elongation. In particular, our data suggests that miR-206 regulates changes in cell adhesion that affect the ability of newly formed somites to adhere to the notochord as well as to the intersomitic boundaries. Additionally, we show that β-dystroglycan and F-actin expression levels are significantly reduced, suggesting that knockdown of miR-206 levels affects cellular mechanics necessary for cell shape changes and attachments that are required for proper muscle formation. |
| Databáze: | OpenAIRE |
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