1,25(OH)2Vitamin D3 induces elevated expression of the cell cycle inhibitor p18 in a squamous cell carcinoma cell line of the head and neck
| Autor: | Gudrun Hager, Claudia Gedlicka, Birgit Knerer, Wilhelm Gedlicka, Martina Weissenböck, Johannes Kornfehl, Michael Formanek |
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| Rok vydání: | 2006 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Cell Cell Count Biology Resting Phase Cell Cycle Pathology and Forensic Medicine chemistry.chemical_compound Calcitriol Downregulation and upregulation Cell Line Tumor medicine Animals Cyclin-Dependent Kinase Inhibitor p18 Humans Cyclin-Dependent Kinase Inhibitor p19 Cell Proliferation Cell growth G1 Phase Cell cycle Cell sorting Molecular biology Neoplasm Proteins Tongue Neoplasms medicine.anatomical_structure Otorhinolaryngology Epidermoid carcinoma chemistry Head and Neck Neoplasms Cell culture Carcinoma Squamous Cell Periodontics Rabbits Oral Surgery Growth inhibition Cyclin-Dependent Kinase Inhibitor p27 |
| Zdroj: | Journal of Oral Pathology and Medicine. 35:472-478 |
| ISSN: | 1600-0714 0904-2512 |
| DOI: | 10.1111/j.1600-0714.2006.00407.x |
| Popis: | BACKGROUND: I α,25-Dihydroxyvitamin D 3 [I,25(OH) 2 Vitamin D 3 ] induces growth inhibition in squamous cell carcinoma (SCC) cell lines of the head and neck by arresting the cells in the G0/GI phase of the cell cycle, probably due to an enhanced expression of p21, which could be demonstrated in other cell lines (JPPA, SCC9) before. In SCC25, a SCC cell line isolated from tongue, growth inhibition but no overexpression of p2I was detected. The retinoblastoma gene, as a direct target of G I cyclin-CDK complexes, showed an obvious shift from the hyperphosphorylated to the hypophosphorylated form under I,25(OH) 2 Vitamin D 3 , which indicates that the growth inhibition takes place in the G0/GI phase. To explore the possible pathway of growth inhibition in SCC25 we investigated other cell cycle inhibitors (pI8, pI9, p27). METHODS: Synchronized cells were treated with I,25(OH) 2 Vitamin D 3 over 96 h. The cell cycle status and expression of cell cycle-regulating proteins was determined by fluorescence-activated cell sorting (FACS) and Western blotting. RESULTS: An overexpression of pI8 in I,25(OH) 2 Vitamin D 3 vs. ethanol-treated cells was determined until 30 h in SCC25. No influence was detectable on the expression of p27 and pI9. CONCLUSION: One mechanism by which I,25(OH) 2 Vitamin D 3 controls cell growth might be the upregulation of p2I. As p2I was unsusceptible to I,25(OH) 2 Vitamin D 3 in SCC25, other inhibiting proteins were necessary to be tested. The proven upregulation of pI8 seems to be the responsible step for growth inhibition of I,25(OH) 2 Vitamin D 3 in SCC25. |
| Databáze: | OpenAIRE |
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