| Autor: |
Ximena Garate, Gabriel Neiman, Ariel Waisman, Lucía Natalia Moro, Alejandro La Greca, Natalia Lucía Santín Velazque, María Agustina Scarafía, Alan Miqueas Möbbs, Carolina Colli, Sheila Castañeda, Cyntia Aban, Carlos Luzzani, María Clara Hernández Cañás, Nelba Pérez, Gustavo Sevlever, Santiago Gabriel Miriuka |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| DOI: |
10.1101/639906 |
| Popis: |
SummaryPIWI-interacting RNAs (piRNAs) are a class of non-coding RNAs initially thought to be restricted almost exclusively to germ line cells. In recent years, accumulating evidence has demonstrated that piRNAs are actually expressed in somatic cells like pluripotent, neural, cardiac and even cancer cells. However, controversy still remains around the existence and function of somatic piRNAs. Using small RNA-seq samples from H9 pluripotent stem cells differentiated to mesoderm progenitors and cardiomyocytes we identified the expression of 447 piRNAs, of which 241 were detected in pluripotency, 218 in mesoderm and 171 in cardiac cells. The majority of them originated from the sense strand of protein coding and lncRNAs genes in all stages of differentiation, though no evidences for secondary piRNAs (ping-pong loop) were found. Genes hosting piRNAs in cardiac samples were related to critical biological processes in the heart, like contraction and cardiac muscle development. Our results indicate that somatic piRNAs might have a role in fine-tuning the expression of genes involved in the differentiation of pluripotent cells to cardiomyocytes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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