High Mobility Group-Box 1 (HMGB1) levels are increased in amniotic fluid of women with intra-amniotic inflammation-determined preterm birth, and the source may be the damaged fetal membranes
| Autor: | Kara M. Rood, Guomao Zhao, Catalin S. Buhimschi, Emily A. Oliver, Irina A. Buhimschi, Margaret A. Baumbusch, Stephen F. Thung |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Lipopolysaccharides 0301 basic medicine Amniotic fluid Receptor for Advanced Glycation End Products Immunology Extraembryonic Membranes Gestational Age chemical and pharmacologic phenomena Chorioamnionitis HMGB1 Biochemistry Article Proinflammatory cytokine Andrology Young Adult 03 medical and health sciences 0302 clinical medicine Pregnancy Humans Immunology and Allergy Medicine HMGB1 Protein Molecular Biology Cellular localization Immunoassay Fetus 030219 obstetrics & reproductive medicine biology Interleukin-6 business.industry Infant Newborn Gestational age Hematology Amniotic Fluid medicine.disease Immunohistochemistry 030104 developmental biology In utero biology.protein Premature Birth Female business |
| Zdroj: | Cytokine. 81:82-87 |
| ISSN: | 1043-4666 |
| DOI: | 10.1016/j.cyto.2016.02.013 |
| Popis: | Background High Mobility Group Box-1 (HMGB1) is considered a prototype alarmin molecule. Upon its extracellular release, HMGB1 engages pattern recognition receptors and the Receptor for Advanced Glycation End-products (RAGE) followed by an outpouring of inflammatory cytokines, including interleukin (IL)-6. Methods We assayed the amniotic fluid (AF) levels of HMGB1 and IL-6 in 255 women that either had a normal pregnancy outcome or delivered preterm. Immunohistochemistry on fetal membranes was used for cellular localization and validation of immunoassay findings. HMGB1 also was analyzed in amniochorion tissue explants subjected to endotoxin. Results AF HMGB1 levels are not gestational age regulated but are increased in women with intra-amniotic inflammation and preterm birth. The likely source is the damaged amniochorion, as demonstrated by immunohistochemistry and explant experiments. Conclusions Our research supports a role for HMGB1 in the inflammatory response leading to preterm birth. As a delayed phase cytokine, in utero exposure to elevated AF HMGB1 levels may have an impact on the newborn beyond the time of birth. |
| Databáze: | OpenAIRE |
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