The sucrose–trehalose 6-phosphate (Tre6P) nexus: specificity and mechanisms of sucrose signalling by Tre6P

Autor: Regina Feil, Umesh P Yadav, Patrick Giavalisco, Hans-Michael Hubberten, John E. Lunn, Guangyou Duan, Petronia Carillo, Maria Piques, Mark Stitt, Dirk Walther, Alexander Ivakov
Rok vydání: 2014
Předmět:
0106 biological sciences
Sucrose
Arabidopsis thaliana
Physiology
Phosphatase
Arabidopsis
Gene Expression
translation
Plant Science
Cycloheximide
Sensitivity and Specificity
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Gene Expression Regulation
Plant
Hexose
signalling
Sugar
Hexoses
030304 developmental biology
2. Zero hunger
chemistry.chemical_classification
0303 health sciences
Deoxyadenosines
biology
Escherichia coli Proteins
Trehalose
trehalose-phosphate synthase
Fructose
Plants
Genetically Modified
trehalose-phosphate phosphatase
biology.organism_classification
Phosphoric Monoester Hydrolases
trehalose-6-phosphate
chemistry
Biochemistry
Glucosyltransferases
Seedlings
Sugar Phosphates
Oxidation-Reduction
Signal Transduction
Research Paper
010606 plant biology & botany
Zdroj: Journal of Experimental Botany
ISSN: 1460-2431
0022-0957
DOI: 10.1093/jxb/ert457
Popis: Summary Trehalose-6-phosphate is a signal of sucrose status in plants and forms part of a homeostatic mechanism that maintains sucrose levels within a range that is appropriate for the cell type and stage of development.
Trehalose 6-phosphate (Tre6P), the intermediate of trehalose biosynthesis, has a profound influence on plant metabolism, growth, and development. It has been proposed that Tre6P acts as a signal of sugar availability and is possibly specific for sucrose status. Short-term sugar-feeding experiments were carried out with carbon-starved Arabidopsis thaliana seedlings grown in axenic shaking liquid cultures. Tre6P increased when seedlings were exogenously supplied with sucrose, or with hexoses that can be metabolized to sucrose, such as glucose and fructose. Conditional correlation analysis and inhibitor experiments indicated that the hexose-induced increase in Tre6P was an indirect response dependent on conversion of the hexose sugars to sucrose. Tre6P content was affected by changes in nitrogen status, but this response was also attributable to parallel changes in sucrose. The sucrose-induced rise in Tre6P was unaffected by cordycepin but almost completely blocked by cycloheximide, indicating that de novo protein synthesis is necessary for the response. There was a strong correlation between Tre6P and sucrose even in lines that constitutively express heterologous trehalose-phosphate synthase or trehalose-phosphate phosphatase, although the Tre6P:sucrose ratio was shifted higher or lower, respectively. It is proposed that the Tre6P:sucrose ratio is a critical parameter for the plant and forms part of a homeostatic mechanism to maintain sucrose levels within a range that is appropriate for the cell type and developmental stage of the plant.
Databáze: OpenAIRE
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