Effect of weightlessness on colloidal particle transport and segregation in self-organising microtubule preparations
| Autor: | James Tabony, Nicolas Glade, Nathalie Rigotti, Sandra Cortès |
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| Přispěvatelé: | Institut de Biosciences et de Biotechnologies de Grenoble (ex-IRTSV) (BIG), Institut National de la Santé et de la Recherche Médicale (INSERM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Duperray, Alain, Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut National de la Santé et de la Recherche Médicale (INSERM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Grenoble Alpes (UGA) |
| Rok vydání: | 2007 |
| Předmět: |
Indoles
Diffusion MESH: Gravitation MESH: Tubulin Microtubules Biochemistry MESH: Rotation 0302 clinical medicine Models Tubulin MESH: Animals Particle density Cytoskeleton MESH: Indoles Microscopy 0303 health sciences MESH: Microtubules Chemistry Weightlessness MESH: Polystyrenes Brain MESH: Diffusion MESH: Fluorescent Dyes Microspheres MESH: Cattle Classical mechanics MESH: Colloids Gravitation Gravity (chemistry) Rotation MESH: Biological Transport MESH: Microspheres Biophysics MESH: Microscopy Electron MESH: Space Flight [SDV.BC]Life Sciences [q-bio]/Cellular Biology Electron Models Biological Chromosomes MESH: Brain 03 medical and health sciences Microtubule Reaction–diffusion system Animals Colloids MESH: Weightlessness Simulation [SDV.BC] Life Sciences [q-bio]/Cellular Biology Weightlessness Simulation Fluorescent Dyes 030304 developmental biology Organic Chemistry MESH: Models Biological Biological Transport Space Flight Biological Microscopy Electron Colloidal particle Polystyrenes MESH: Chromosomes Cattle 030217 neurology & neurosurgery |
| Zdroj: | Biophysical Chemistry Biophysical Chemistry, Elsevier, 2007, 127 (3), pp.172-80. ⟨10.1016/j.bpc.2007.01.010⟩ Biophysical Chemistry, 2007, 127 (3), pp.172-80. ⟨10.1016/j.bpc.2007.01.010⟩ |
| ISSN: | 0301-4622 |
| DOI: | 10.1016/j.bpc.2007.01.010 |
| Popis: | International audience; Weightlessness is known to effect cellular functions by as yet undetermined processes. Many experiments indicate a role of the cytoskeleton and microtubules. Under appropriate conditions in vitro microtubule preparations behave as a complex system that self-organises by a combination of reaction and diffusion. This process also results in the collective transport and organisation of any colloidal particles present. In large centimetre-sized samples, self-organisation does not occur when samples are exposed to a brief early period of weightlessness. Here, we report both space-flight and ground-based (clinorotation) experiments on the effect of weightlessness on the transport and segregation of colloidal particles and chromosomes. In centimetre-sized containers, both methods show that a brief initial period of weightlessness strongly inhibits particle transport. In miniature cell-sized containers under normal gravity conditions, the particle transport that self-organisation causes results in their accumulation into segregated regions of high and low particle density. The gravity dependence of this behaviour is strongly shape dependent. In square wells, neither self-organisation nor particle transport and segregation occur under conditions of weightlessness. On the contrary, in rectangular canals, both phenomena are largely unaffected by weightlessness. These observations suggest, depending on factors such as cell and embryo shape, that major biological functions associated with microtubule driven particle transport and organisation might be strongly perturbed by weightlessness. |
| Databáze: | OpenAIRE |
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