Natural variation and dosage of the HEI10 meiotic E3 ligase control Arabidopsis crossover recombination
| Autor: | Charles J Underwood, Liliana Ziolkowska, Emma J. Lawrence, F. Chris H. Franklin, Marina Martinez-Garcia, Christophe Lambing, Robert A. Martienssen, Kyuha Choi, Ian R. Henderson, Catherine H Griffin, Piotr Ziółkowski |
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| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
Coefficient of coincidence Euchromatin Chromosomal Proteins Non-Histone Crossover Quantitative Trait Loci Arabidopsis Gene Dosage Biology Genetic recombination 03 medical and health sciences Meiosis Genetics Ectopic recombination Amino Acid Sequence Crossing Over Genetic Recombination Genetic Sequence Homology Amino Acid Arabidopsis Proteins biology.organism_classification 030104 developmental biology Recombination Developmental Biology Research Paper |
| Zdroj: | Genes & Development |
| ISSN: | 1549-5477 0890-9369 |
| DOI: | 10.1101/gad.295501.116 |
| Popis: | During meiosis, homologous chromosomes undergo crossover recombination, which creates genetic diversity and balances homolog segregation. Despite these critical functions, crossover frequency varies extensively within and between species. Although natural crossover recombination modifier loci have been detected in plants, causal genes have remained elusive. Using natural Arabidopsis thaliana accessions, we identified two major recombination quantitative trait loci (rQTLs) that explain 56.9% of crossover variation in Col×Ler F2 populations. We mapped rQTL1 to semidominant polymorphisms in HEI10, which encodes a conserved ubiquitin E3 ligase that regulates crossovers. Null hei10 mutants are haploinsufficient, and, using genome-wide mapping and immunocytology, we show that transformation of additional HEI10 copies is sufficient to more than double euchromatic crossovers. However, heterochromatic centromeres remained recombination-suppressed. The strongest HEI10-mediated crossover increases occur in subtelomeric euchromatin, which is reminiscent of sex differences in Arabidopsis recombination. Our work reveals that HEI10 naturally limits Arabidopsis crossovers and has the potential to influence the response to selection. |
| Databáze: | OpenAIRE |
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