Cell-free synthesis of Rauscher murine leukemia virus 'gag' and 'env' gene products from separate cellular mRNA species
| Autor: | Edwin C. Murphy, Ralph B. Arlinghaus, Daniel Campos |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Antiserum
Messenger RNA Cell-Free System biology viruses RNA Translation (biology) biology.organism_classification Rauscher Virus Molecular biology Virus Cell Line Viral Proteins Protein Biosynthesis Virology Murine leukemia virus Agarose gel electrophoresis RNA Messenger Protein Precursors Gene Glycoproteins |
| Zdroj: | Virology. 93:293-302 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/0042-6822(79)90234-4 |
| Popis: | RNA from cells infected with Rauscher murine leukemia virus (R-MuLV) has been translated in an mRNA-dependent cell-free protein synthesizing system. It was found that a cellular RNA species of about 35 S in size codes for polypeptides of approximately 65,000 MW (Pr65 gag ) and 200,000 MW (Pr200 gag ) which are immunoprecipitable with antisera directed against the R-MuLV gag proteins p30, p15, p12, and p10. The methionine-containing-tryptic peptides of the 65,000 MW polypeptide translated from cellular 35 S RNA were identical to those of authentic Pr65 gag . Translation of RNA in the 25–35 S size class suggests that while Pr65 gag can be translated by RNA throughout this size range, Pr200 gag-pol translation is restricted to mRNA which sediments at 35 S. Antiserum directed against the R-MuLV envelope protein gp69/71 recognized a polypeptide of 68,000 MW, designated Pr68 env , which was coded for by RNA which sedimented at about 22 S in sucrose gradients and which had a minimum size of about 1.25 × 10 6 daltons as estimated by agarose gel electrophoresis. Tryptic maps of Pr68 env showed it to contain all of the methionine-labeled tryptic peptides and most of the tyrosine-containing tryptic peptides characteristic of gPr90 env the authentic R-MuLV glycosylated envelope precursor. |
| Databáze: | OpenAIRE |
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