NuA3 HAT antagonizes the Rpd3S and Rpd3L HDACs to optimize mRNA and lncRNA expression dynamics
| Autor: | Ahyoung Choi, Hyeonju Woo, Ji Hyung Kim, Sanghyuk Lee, So Dam Ha, Ji Eun Lee, Yukyung Jun, TaeSoo Kim, Ji-Hyun Kim, Bo Bae Lee, Woojin Jeong, Chae Young Yoon |
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| Rok vydání: | 2020 |
| Předmět: |
Saccharomyces cerevisiae Proteins
AcademicSubjects/SCI00010 Saccharomyces cerevisiae Histone Deacetylases 03 medical and health sciences Histone H3 Gene Expression Regulation Fungal Genetics RNA Messenger Histone Acetyltransferases 030304 developmental biology 0303 health sciences Binding Sites biology Chromatin binding Gene regulation Chromatin and Epigenetics 030302 biochemistry & molecular biology Methyltransferases Histone acetyltransferase Chromatin Cell biology Histone PHD finger biology.protein H3K4me3 RNA Long Noncoding Histone deacetylase Protein Binding |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gkaa781 |
| Popis: | In yeast, NuA3 histone acetyltransferase (NuA3 HAT) promotes acetylation of histone H3 lysine 14 (H3K14) and transcription of a subset of genes through interaction between the Yng1 plant homeodomain (PHD) finger and H3K4me3. Although NuA3 HAT has multiple chromatin binding modules with distinct specificities, their interdependence and combinatorial actions in chromatin binding and transcription remain unknown. Modified peptide pulldown assays reveal that the Yng1 N-terminal region is important for the integrity of NuA3 HAT by mediating the interaction between core subunits and two methyl-binding proteins, Yng1 and Pdp3. We further uncover that NuA3 HAT contributes to the regulation of mRNA and lncRNA expression dynamics by antagonizing the histone deacetylases (HDACs) Rpd3S and Rpd3L. The Yng1 N-terminal region, the Nto1 PHD finger and Pdp3 are important for optimal induction of mRNA and lncRNA transcription repressed by the Set2-Rpd3S HDAC pathway, whereas the Yng1 PHD finger–H3K4me3 interaction affects transcriptional repression memory regulated by Rpd3L HDAC. These findings suggest that NuA3 HAT uses distinct chromatin readers to compete with two Rpd3-containing HDACs to optimize mRNA and lncRNA expression dynamics. |
| Databáze: | OpenAIRE |
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