Recombinant C3adesArg/acylation stimulating protein (ASP) is highly bioactive: a critical evaluation of C5L2 binding and 3T3-L1 adipocyte activation
| Autor: | David Kalant, Danny Gauvreau, Wei Cui, Marc Lapointe, Katherine Cianflone |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Protamine sulfate
Immunology Adipose tissue CHO Cells Biology law.invention chemistry.chemical_compound Acylation stimulating protein Mice Cricetulus law Adipocyte 3T3-L1 Cells Cricetinae medicine Adipocytes Viperidae Animals Humans Receptor Molecular Biology Receptor Anaphylatoxin C5a Mice Knockout Fatty Acids Transfection Recombinant Proteins Biochemistry chemistry GPR77 Recombinant DNA Complement C3a Receptors Chemokine medicine.drug Protein Binding |
| Zdroj: | Molecular immunology. 46(16) |
| ISSN: | 1872-9142 |
| Popis: | C5L2 is a recently identified receptor for C5a/C5adesArg, C3a and C3adesArg (ASP). C5a/C5adesArg bind with high affinity, with no identified activation. By contrast, some studies demonstrate C3a/ASP binding/activation to C5L2; others do not. Our aim is to critically evaluate ASP/C3adesArg-C5L2 binding and bioactivity. Cell-associated fluorescent-ASP (Fl-ASP) binding to C5L2 increased from transiently transfected |
| Databáze: | OpenAIRE |
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