C5b‐8 Step Lysis of Swine Endothelial Cells by Human Complement and Functional Feature of Transfected CD59
| Autor: | Misako Matsumoto, Terado A, Shuji Miyagawa, Shoki Mikata, R Shirakura, Hikaru Matsuda, Michiyo Hatanaka, Tsukasa Seya, Shigeharu Nagasawa |
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| Rok vydání: | 1996 |
| Předmět: |
Cytotoxicity
Immunologic Lysis Swine Immunology Dose-Response Relationship Immunologic CD59 Antigens chemical and pharmacologic phenomena CD59 Biology Transfection Umbilical vein Cell Line Membrane Cofactor Protein Antigens CD Complementary DNA Animals Humans Complement Inactivator Proteins Membrane Glycoproteins CD55 Antigens CD46 Complement System Proteins General Medicine Molecular biology In vitro Endothelial stem cell Endothelium Vascular |
| Zdroj: | Scandinavian Journal of Immunology. 43:361-366 |
| ISSN: | 1365-3083 0300-9475 |
| Popis: | The authors established several swine endothelial cell (SEC) lines expressing human CD59 by transfection of cDNA, and assessed the function of the transfectant molecules in comparison with those of membrane cofactor protein (MCP) and decay-accelerating factor (DAF) in an in vitro hyperacute rejection model of swine to human discordant xenograft. At the usual expression rate, DAF and MCP protected SEC from human complement mediated cell lysis, but CD59 did not block human complement attack on SEC. However, CD59 protects SEC from cell lysis when sufficiently expressed as in human umbilical vein (HUVEC). The authors examined why CD59 needed so many molecules to protect human complement-mediated SEC lysis and found that SEC underwent lysis by human C5b-8. The degree of C5b-8 step lysis of SEC was approximately 70% of the total activation (C5b-9). Additionally, CD59 protected human complement activities less efficiently at the C5b-8 step than at the C9-step. Therefore, to overcome human complement mediated SEC lysis, C8 activity must be inhibited by dense expression of CD59. |
| Databáze: | OpenAIRE |
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