T-lymphocytes are Directly Involved in the Clinical Expression of Migratory Circinate Erythema in Epidermolysis Bullosa Simplex Patients
| Autor: | Giovanna Zambruno, Andrea Cavani, Monica Pascucci, Andrea Diociaiuti, Naomi De Luca, Teresa Carbone, Daniele Castiglia, May El Hachem |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte CD4-Positive T-Lymphocytes Cellular immunity Pathology medicine.medical_specialty Erythema Biopsy Immunoglobulins Apoptosis Dermatology CD8-Positive T-Lymphocytes Lesion Epidermolysis bullosa simplex Antigens CD In Situ Nick-End Labeling Humans Medicine Genetic Predisposition to Disease Lectins C-Type Child Skin Immunity Cellular Membrane Glycoproteins integumentary system Epidermis (botany) medicine.diagnostic_test business.industry Papillary dermis Infant Newborn General Medicine medicine.disease Immunohistochemistry Keratin 5 Phenotype Child Preschool Epidermolysis Bullosa Simplex Mutation Skin biopsy Keratin-5 Female medicine.symptom business Biomarkers |
| Zdroj: | Acta Dermato Venereologica. 94:307-311 |
| ISSN: | 0001-5555 |
| Popis: | Epidermolysis bullosa simplex with migratory circinate erythema (EBS-MCE) is a rare EBS subtype characterised by migratory blistering lesions that resolve with brownish pigmentation. It is caused by a recurrent readthrough mutation, c.1649delG, in the tail of keratin 5. Here, we report a child with EBS-MCE and investigated the immunologic mechanisms underlying the migratory lesions in this patient. A skin biopsy from the patient from an active border of an erythematous lesion was used for the immunohistochemical characterisation of the inflammatory infiltrate and for TUNEL assay to detect apoptotic cells. We found abundant CD4+ and CD8+ T lymphocytes infiltrating the papillary dermis and lining the dermal-epidermal junction. A number of these cells expressed the activation marker CD69. CD83+ dendritic cells were present both in the epidermis and papillary dermis. Finally, TUNEL staining showed apoptosis of basal and suprabasal keratinocytes. These findings suggest a critical role of the cellular immunity in determining the EBS-MCE phenotype. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|