Proteomic Analysis and Functional Studies of Baicalin on Proteins Associated with Skin Cancer
| Autor: | Dan Li, Nabiha Yusuf, Erin M. Burns, Wei Min, Bing-jiang Lin, Xiuqin Yu, Dan Luo |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Proteomics Skin Neoplasms Human skin Apoptosis Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Tumor Cells Cultured Humans Functional studies Molecular Targeted Therapy RNA Small Interfering Ultraviolet radiation Cell Proliferation Flavonoids integumentary system Cell Cycle General Medicine medicine.disease Molecular biology Antineoplastic Agents Phytogenic 030104 developmental biology Cell Transformation Neoplastic Complementary and alternative medicine chemistry 030220 oncology & carcinogenesis Cancer research RNA Interference Skin cancer Baicalin Nucleophosmin Phytotherapy Scutellaria baicalensis |
| Zdroj: | The American journal of Chinese medicine. 45(3) |
| ISSN: | 1793-6853 |
| Popis: | Abundant evidence supports the key role of ultraviolet radiation (UVR) in skin cancer development. The human skin, especially the epidermal layer, is the main defense against UV radiation. Baicalin is a major bioactive component of Scutellaria baicalensis Georgi, a plant which has been found to exhibit antitumor activity. The anticarcinogenic mechanism of baicalin is not completely understood. We have reported that baicalin inhibited UVB-induced photo-damage and apoptosis in HaCaT cells (human skin keratinocytes). The aim of the present study is to investigate the cellular gene targets responsible for baicalin’s antitumor activity by performing two-dimensional electrophoresis liquid chromatography-mass spectrometry/mass spectrometry (2-DE LC-MS/MS) with HaCaT cells following UVB and baicalin exposure. Two-DE for protein separation was performed, followed by matrix-assisted laser desorption/ionization mass spectrometry and database searches. Nucleophosmin (NPM)-specific siRNA was designed and synthesized, and the small interfering RNA was transfected into skin squamous cancer A431 cells to knockdown the NPM expression. Proliferation and cell cycle status were assessed by CCK8 and flow cytometric analyses, respectively. We have identified 38 protein spots that are differentially expressed in HaCaT cells exposed to baicalin and/or UVB irradiation These proteins are involved in detoxification, proliferation, metabolism, cytoskeleton and motility. In particular, we found several proteins that have been linked to tumor progression and resistance, such as NPM. Baicalin treatment reduced the cellular proliferation rate and induced arrest during the S-phase of the cell cycle in A431 cells. NPM1 silencing significantly enhanced the effect of baicalin. Our data indicated that baicalin results in the significant inhibition of tumor growth in the A431 cell line, which may be associated with the regulation of the NPM gene expression. |
| Databáze: | OpenAIRE |
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