Microarray analysis reveals overexpression of IBSP in human carotid plaques
| Autor: | H Ayari, G Bricca |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Pathology medicine.medical_specialty Carbonic Anhydrase II Matrix gla protein medicine Humans Integrin-Binding Sialoprotein Carotid Stenosis Osteopontin RNA Messenger Vascular Calcification Aged Oligonucleotide Array Sequence Analysis Aged 80 and over biology Microarray analysis techniques General Medicine Middle Aged medicine.disease Plaque Atherosclerotic Up-Regulation Arterial calcification Atheroma biology.protein Osteocalcin Female Osteonectin Transcriptome Calcification |
| Zdroj: | Advances in medical sciences. 57(2) |
| ISSN: | 1898-4002 |
| Popis: | Purpose Vascular calcification was considered to be a passive, degenerative, and end-stage process of vascular disease. However, bone associated proteins such as bone morphogenetic proteins, osteopontin, osteonectin, osteocalcin, and matrix Gla protein (MGP) have been found in the calcified atherosclerotic lesions. We studied by microarray analysis whether intact tissue and carotid plaque from the same patient differ in transcriptional profiling in response to arterial calcification. Material and Methods mRNA gene expression was measured by an Affymetrix GeneChip Human Gene 1.0 ST arrays (Affymetrix, Santa Clara, CA, USA) using RNA prepared from 68 specimens of endarterectomy from 34 patients. Results Integrin-binding sialoprotein (IBSP) was found to be differentially expressed. IBSP mRNA is over expressed in atheroma plaque (3.74 fold, p = 1.41E-09) in an intraindividual comparison. Besides, Carbonic anhydrase II (CA2) which known to be a putative calcification inhibitory molecule is over expressed more than 1.7 fold in carotid plaque (p = 1.26E-06). Conclusion Although further evidence is needed, our results support previously available data. To our knowledge, this is the first report comparing gene expression between intact arterial tissue and carotid plaque using microarray analysis in order to identify calcification related genes. We suggest that plaques show a more pronounced induction of IBSP that may cause arterial calcification. |
| Databáze: | OpenAIRE |
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