Proton Magnetic Resonance Studies of Methionine Enkephalin
| Autor: | Peter Tonui, Angela Miller, Dave Powell, S. S. Toporek, Thomas J. Manning |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
inorganic chemicals
Magnetic Resonance Spectroscopy Enkephalin Cations Divalent Protein Conformation Enkephalin Methionine Strong interaction Biophysics Analytical chemistry Protonation Ligands Biochemistry chemistry.chemical_compound Molecular Biology Acetic Acid Binding Sites Methionine Cell Biology Ethylenes Methionine enkephalin Solution phase Proton magnetic resonance Crystallography chemistry Thermodynamics Diamagnetism Copper |
| Zdroj: | Biochemical and Biophysical Research Communications. 226:796-800 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1996.1431 |
| Popis: | In the literature there exist two conflicting pieces of data concerning the thermodynamics of the Cu(2+)-methionine enkephalin complex. One study utilized 1H nuclear magnetic resonance (NMR) to study the solution phase Cu(2+)-enkephalin binding and reported a strong interaction over a wide pH range. Another study utilized potentiometry to measure a log beta 101 of 4.6, a value representative of a weak metal-ligand complex. In order to resolve this discrepancy a 300 MHz 1H NMR is used to study the protonation sequence of methionine enkaphalin, a bioactive peptide, and its complexation with seven diamagnetic metals (Ba2+, Ca2+, Zn2+, La2+, Mg2+, Sr2+ and Cd2+). |
| Databáze: | OpenAIRE |
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