In vitro inhibition by gossypol of oxidoreductases from human tissues
| Autor: | C Burgos, Antonio Blanco, Leonor E. Rovai, Gerez de Burgos Nm |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Male
Dehydrogenase Biology Biochemistry Malate dehydrogenase Isozyme Cofactor chemistry.chemical_compound Glutamate Dehydrogenase Malate Dehydrogenase Lactate dehydrogenase Humans Infertility Male Pharmacology L-Lactate Dehydrogenase Myocardium Glutamate dehydrogenase Gossypol Spermatozoa Isoenzymes Liver chemistry biology.protein Branched-chain alpha-keto acid dehydrogenase complex |
| Zdroj: | Biochemical Pharmacology. 35:801-804 |
| ISSN: | 0006-2952 |
| Popis: | The effect of gossypol, a polyphenolic compound with antifertility action on human males, has been investigated on the following oxidoreductases purified from human tissues: lactate dehydrogenase (EC 1.1.1.27) isozymes 1 or B4 from heart, 5 or A4 from liver and X or C4 from spermatozoa; malate dehydrogenase (EC 1.1.1.37) mitochondrial and "soluble" isozymes from heart and NADP-glutamate dehydrogenase (EC 1.4.1.4) from liver. Gossypol proved to be a powerful inhibitor of the six enzymes studied. For all of them, inhibition was of the competitive type with respect to the coenzyme and non-competitive in relation to substrate. The lowest ki values were shown for lactate dehydrogenase isozyme 1 or B4 and for the two isozymes of malate dehydrogenase. Results did not show selectivity of gossypol for the sperm-specific isozyme X or C4 of lactate dehydrogenase. |
| Databáze: | OpenAIRE |
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