Role of kisspeptin on cell proliferation and steroidogenesis in luteal cells in vitro and in vivo
| Autor: | Hsin Yi Chiu, Chia Sheng Chang, Ya Yun Chien, Leang-Shin Wu, De-Shien Jong, Chi Ming Chiang, Chih-Hsien Chiu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
endocrine system
Cell Survival Cell Taiwan Gene Expression 030204 cardiovascular system & hematology Luteal phase Polymerase Chain Reaction Andrology 03 medical and health sciences 0302 clinical medicine In vivo Luteal Cells Animals Medicine RNA Messenger Protein kinase A Receptor Cell Proliferation Kisspeptins business.industry Cell growth Goats General Medicine medicine.anatomical_structure Cell culture 030220 oncology & carcinogenesis Female business Corpus luteum |
| Zdroj: | Journal of the Chinese Medical Association. 84:389-399 |
| ISSN: | 1726-4901 |
| DOI: | 10.1097/jcma.0000000000000508 |
| Popis: | BACKGROUND Kisspeptin (KISS1) and kisspeptin receptor (KISS1R) are essential gatekeepers of the reproductive system. The functions of KISS1 and KISS1R in corpus luteal cells remain ambiguous. The objective was to observe normal physiologic functions of corpus luteal cells in vivo and clarify the functions of KISS1 in vitro. METHODS We conducted an in vivo observation of cellular patterns as well as the levels of steroidogenic enzymes and KISS1/KISS1R in corpus luteal cells obtained from female crossbred Taiwan native goats in the estrous cycle; the observation was performed using hematoxylin and eosin and immunohistochemistry staining. Subsequently, we used kisspeptin-10 (Kp-10) to stimulate temperature sensitive-caprine luteal cell line (ts-CLC-D) cells to investigate the progesterone (P4) levels, steroidogenic messenger RNA (mRNA)/protein levels, cell survival rate, intracellular Ca2+ concentration, and cell proliferation-related mRNA/protein levels in the mitogen-activated protein kinase pathway in vitro by applying immunofluorescence staining, Western blotting, 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide assay, and real-time polymerase chain reaction. RESULTS We observed the presence of proteins and mRNAs for STAR, CYP11A1, HSD3B, KISS1, and KISS1R in the corpus luteal cells from goats in vivo. In vitro, the addition of Kp-10 reduced the P4 levels (p < 0.01) and increased cell proliferation (p < 0.05) of the ts-CLC-D cells. Furthermore, we found that the levels of proteins and mRNA for STAR, CYP11A1, and HSD3B decreased significantly when Kp-10 was added (p < 0.05). However, adding Kp-10 did not affect the mRNA levels for PLCG2, DAG1, PRKCA, KRAS, RAF1, MAP2K1, MAP2K2, MAPK3, MAPK1, and MAPK14. CONCLUSION We determined that KISS1 could affect the P4 levels, steroidogenesis, and cell proliferation in luteal cells. However, further research is required to clarify how KISS1 regulates proliferation and steroid production in luteal cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|