Common Regulatory Targets of NFIA, NFIX and NFIB during Postnatal Cerebellar Development
| Autor: | Alexander S. Brown, Lauren P. Shapiro, Matthew P. Scott, Alexandra Essebier, Raul Ayala Davila, Tracey J. Harvey, Michael Piper, Mikael Bodén, Brandon J. Wainwright, James Fraser, Peter Penzes, Oressia Zalucki, Danyon Harkins, Richard M. Gronostajski |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Neurogenesis Biology Article 03 medical and health sciences Mice 0302 clinical medicine Pregnancy Cerebellum Transcriptional regulation Animals Transcription factor 030304 developmental biology Mice Knockout 0303 health sciences Nuclear factor I NFIX Cell biology Mice Inbred C57BL NFI Transcription Factors Neurology NFIB Animals Newborn NFIA biology.protein Chromatin Immunoprecipitation Sequencing Female Neurology (clinical) Neural development 030217 neurology & neurosurgery |
| Zdroj: | Cerebellum |
| ISSN: | 1473-4230 |
| Popis: | Transcriptional regulation plays a central role in controlling neural stem and progenitor cell proliferation and differentiation during neurogenesis. For instance, transcription factors from the nuclear factor I (NFI) family have been shown to co-ordinate neural stem and progenitor cell differentiation within multiple regions of the embryonic nervous system, including the neocortex, hippocampus, spinal cord and cerebellum. Knockout of individual Nfi genes culminates in similar phenotypes, suggestive of common target genes for these transcription factors. However, whether or not the NFI family regulates common suites of genes remains poorly defined. Here, we use granule neuron precursors (GNPs) of the postnatal murine cerebellum as a model system to analyse regulatory targets of three members of the NFI family: NFIA, NFIB and NFIX. By integrating transcriptomic profiling (RNA-seq) of Nfia- and Nfix-deficient GNPs with epigenomic profiling (ChIP-seq against NFIA, NFIB and NFIX, and DNase I hypersensitivity assays), we reveal that these transcription factors share a large set of potential transcriptional targets, suggestive of complementary roles for these NFI family members in promoting neural development. |
| Databáze: | OpenAIRE |
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