Persistent and polarized global actin flow is essential for directionality during cell migration

Autor: Brian Stramer, Andrei Luchici, Robert G. Endres, Jan Müller, Will Wood, Mubarik Burki, Stefania Marcotti, Eduardo Serna-Morales, Michael Sixt, Lawrence Yolland, Andrew D. Davidson, Linus J. Schumacher, John Robert Davis, Fiona N Kenny, Mark Miodownik
Rok vydání: 2019
Předmět:
Keratinocytes
Embryo
Nonmammalian
Hemocytes
ADHESION
PROTRUSION
Mechanotransduction
Cellular
0302 clinical medicine
Cell Movement
Genes
Reporter
Cell polarity
Myosin
Mechanotransduction
11 Medical and Health Sciences
Zebrafish
0303 health sciences
Chemistry
Cell Polarity
Gene Expression Regulation
Developmental
Cell migration
MEMBRANE TENSION
LAMELLIPODIUM
Cell biology
Drosophila melanogaster
Cell Tracking
030220 oncology & carcinogenesis
Life Sciences & Biomedicine
Cofilin 1
Leading edge
Green Fluorescent Proteins
Primary Cell Culture
Motility
Myosins
Article
03 medical and health sciences
MOTILITY
MACROPHAGE-MIGRATION
Animals
Directionality
Actin
030304 developmental biology
Science & Technology
Macrophages
MYOSIN-II
Cell Biology
06 Biological Sciences
Actins
Luminescent Proteins
FILAMENTOUS ACTIN
RETROGRADE FLOW
CONTACT INHIBITION
Developmental Biology
Zdroj: Nature cell biology
Yolland, L, Burki, M, Marcotti, S, Luchici, A, Kenny, F N, Davis, J R, Serna-Morales, E, Müller, J, Sixt, M, Davidson, A, Wood, W, Schumacher, L J, Endres, R G, Miodownik, M & Stramer, B M 2019, ' Persistent and polarized global actin flow is essential for directionality during cell migration ', Nature Cell Biology, vol. 21, no. 11, pp. 1370–1381 . https://doi.org/10.1038/s41556-019-0411-5
Yolland, L, Burki, M, Marcotti, S, Luchici, A, Kenny, F N, Davis, J R, Serna-Morales, E, Müller, J, Sixt, M, Davidson, A, Wood, W, Schumacher, L J, Endres, R G, Miodownik, M & Stramer, B M 2019, ' Persistent and polarised global actin flow is essential for directionality during cell migration ', Nature Cell Biology, vol. 21, no. 11, pp. 1370–1381 . https://doi.org/10.1038/s41556-019-0411-5
Nature Cell Biology
ISSN: 1476-4679
1465-7392
DOI: 10.1038/s41556-019-0411-5
Popis: Cell migration is hypothesized to involve a cycle of behaviours beginning with leading edge extension. However, recent evidence suggests that the leading edge may be dispensable for migration, raising the question of what actually controls cell directionality. Here, we exploit the embryonic migration of Drosophila macrophages to bridge the different temporal scales of the behaviours controlling motility. This approach reveals that edge fluctuations during random motility are not persistent and are weakly correlated with motion. In contrast, flow of the actin network behind the leading edge is highly persistent. Quantification of actin flow structure during migration reveals a stable organization and asymmetry in the cell-wide flowfield that strongly correlates with cell directionality. This organization is regulated by a gradient of actin network compression and destruction, which is controlled by myosin contraction and cofilin-mediated disassembly. It is this stable actin-flow polarity, which integrates rapid fluctuations of the leading edge, that controls inherent cellular persistence.
Databáze: OpenAIRE
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