Preparation and purification of microplasmin
| Autor: | Hua Lin Wu, Guey Yueh Shi, Myron L. Bender |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Gel electrophoresis
Multidisciplinary Chromatography biology Plasmin Polyacrylamide Size-exclusion chromatography Active site Trypsin Chromatography Affinity Peptide Fragments Molecular Weight chemistry.chemical_compound Affinity chromatography chemistry medicine biology.protein Humans Fibrinolysin Enzyme kinetics Research Article medicine.drug |
| Zdroj: | Proceedings of the National Academy of Sciences. 84:8292-8295 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.84.23.8292 |
| Popis: | A catalytically active, human microplasmin was produced by incubation of [Lys]plasmin in buffer at pH 11.0 for up to 12 hr. The microplasmin was purified by affinity chromatography that used lysine-Sepharose and soybean trypsin inhibitor-Sepharose columns. It is homogeneous and pure by electrophoretic analysis in NaDodSO4/polyacrylamide gels and by gel filtration on a Superose 12 column. The molecular weight of the microplasmin determined by NaDodSO4 gel electrophoresis is 29,000 and 26,500 under reducing condition, whereas the molecular weight of native plasmin is 76,500. Microplasmin consists mainly of the ligh (B) chain of native human plasmin and possesses one active site per protein molecule when titrated with p-nitrophenyl p'-guanidinobenzoate. Microplasmin hydrolyzes the peptide substrate NH2-D-Val-Leu-Lys-p-nitroanilide (S-2251) with a Km of 0.361 +/- 0.017 mM and a kcat of 40.3 +/- 3.3 s-1 at pH 7.4 and 37 degrees C, whereas native plasmin has a Km of 0.355 +/- 0.002 mM and a kcat of 27.9 +/- 0.3 s-1 under the same conditions. |
| Databáze: | OpenAIRE |
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