cGMP Signalling Mediates Water Sensation (Hydrosensation) and Hydrotaxis in Caenorhabditis elegans
| Autor: | Jing Jing Wu, Zhaoyu Li, Wei Wang, Chun Ming Su, Hui Liu, Ya Qian Wu, Yan Xue Song, Yao Xu, Ming Hai Ge, Chang Li Ge, Li Wei Qin, Lan Lan Li, Tai Hong Wu, Qiang Zhang, Yuan Hua Chen, Jing Tang, Zheng-Xing Wu |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Sensory Receptor Cells Sensation Sensory system Optogenetics Article Receptors G-Protein-Coupled Agar plate 03 medical and health sciences Animals Mating Caenorhabditis elegans Cyclic GMP Multidisciplinary biology Behavior Animal Ecology Water biology.organism_classification Hedgehog signaling pathway Cell biology 030104 developmental biology Signal transduction Genetic screen Signal Transduction |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Animals have developed the ability to sense the water content in their habitats, including hygrosensation (sensing humidity in the air) and hydrosensation (sensing the water content in other microenvironments) and they display preferences for specific water contents that influence their mating, reproduction and geographic distribution. We developed and employed four quantitative behavioural test paradigms to investigate the molecular and cellular mechanisms underlying sensing the water content in an agar substrate (hydrosensation) and hydrotaxis in Caenorhabditis elegans. By combining a reverse genetic screen with genetic manipulation, optogenetic neuronal manipulation and in vivo Ca2+ imaging, we demonstrate that adult worms avoid the wetter areas of agar plates and hypo-osmotic water droplets. We found that the cGMP signalling pathway in ciliated sensory neurons is involved in hydrosensation and hydrotaxis in Caenorhabditis elegans. |
| Databáze: | OpenAIRE |
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