In vitro adenosine deaminase inhibitory activity of some selected plant extracts and chemical compounds
| Autor: | Özlem Saçan, Aris Tercan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
plant extracts
Deamination chemistry.chemical_compound Adenosine deaminase Deoxyadenosine Health Care Sciences and Services Phosphatase medicine Sağlık Bilimleri ve Hizmetleri Inosine enzyme inhibition IC50 Cancer biology Farmakoloji ve Eczacılık Chemistry Adenosine deaminase enzyme inhibition plant extracts chemical compounds Antioxidant Activity chemical compounds Adenosine In vitro Citrus-Fruits Biochemistry biology.protein Pharmacology and Pharmacy Quercetin medicine.drug |
| Zdroj: | Volume: 50, Issue: 3 283-288 İstanbul Journal of Pharmacy |
| ISSN: | 2587-2087 |
| DOI: | 10.26650/istanbuljpharm.2019.0079 |
| Popis: | Background and Aims: Adenosine deaminase (EC 3.5.4.4, ADA) is distributed in all human tissues. It catalyses the deamina tion of adenosine (deoxyadenosine) to inosine (deoxyinosine) via removal of an amino group. The determination of ADA-inhibition is of extreme significance in the field of fundamental research and clinical diagnosis. In our study, the effects of various plant extracts and chemicals on ADA activity were investigated. Methods: Adenosine deaminase activity was determined spectrophotometrically at 625 nm. Results: The inhibitory activities of the extracts and chemical compounds increased in a dose-dependent manner. Among the plant extracts used, lemon extract was observed to exhibit the highest ADA inhibition activity with an IC50 value of 0.05 +/- 0.001 mg/ml, while quercetin had the highest ADA inhibition (IC50 value of 0.004 +/- 0.0005 mg/mL) among the chemical compounds. All plant extracts and chemical compounds showed ADA inhibition activities. Conclusion: The obtained results indicate that plant extracts and some chemical compounds examined in this study can be a potential source of novel ADA-inhibitors for therapeutics. |
| Databáze: | OpenAIRE |
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