Impact of electronic-cigarette refill liquid on rat testis

Autor:	M. El May, M Lebret, S. El Fazaa, Aicha Jrad-Lamine, Y. Bdiri, Manel Jallouli, N. El Golli, Yosra Dallagi, Dalila Rahali, Nathalie Ba, Jean-Philippe Rosa
Rok vydání:	2016
Předmět:	Male 0301 basic medicine Nicotine medicine.medical_specialty Health Toxicology and Mutagenesis Dehydrogenase Electronic Nicotine Delivery Systems Toxicology medicine.disease_cause law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine law Internal medicine Lactate dehydrogenase Testis medicine Animals Testosterone Rats Wistar L-Lactate Dehydrogenase Sperm Count biology Chemistry Cholesterol side-chain cleavage enzyme Cytochrome P450 Spermatozoa Oxidative Stress 030104 developmental biology Endocrinology biology.protein Electronic cigarette Biomarkers Injections Intraperitoneal 030217 neurology & neurosurgery Oxidative stress medicine.drug
Zdroj:	Toxicology Mechanisms and Methods. 26:417-424
ISSN:	1537-6524 1537-6516
DOI:	10.3109/15376516.2016.1163448
Popis:	Electronic cigarettes (e-cigarettes) are becoming the fashionable alternative to decrease tobacco smoking, although their impact on health has not been fully assessed yet. The present study was designed to compare the impact of e-cigarette refill liquid (e-liquid) without nicotine to e-liquid with nicotine on rat testis. For this purpose, e-liquid with nicotine and e-liquid without nicotine (0.5 mg/kg of body weight) were administered to adult male Wistar rats via the intraperitoneally route during four weeks. Results showed that e-liquid with or without nicotine leads to diminished sperm density and viability, such as a decrease in testicular lactate dehydrogenase activity and testosterone level. Furthermore, quantitative real-time polymerase chain reaction (qRT-PCR) analysis identified a reduction in cytochrome P450 side-chain cleavage (P450 scc) and 17 beta-hydroxysteroid dehydrogenase (17βHSD) mRNA level, two key enzymes of steroidogenesis. Following e-liquid exposure, histopathological examination showed alterations in testis tissue marked by germ cells desquamation, disorganization of the tubular contents of testis and cell deposits in seminiferous tubules. Finally, analysis of oxidative stress status pointed an outbreak of antioxidant enzyme activities such as superoxide dismutase, catalase and gluthatione-S-transferase, as well as an important increase in sulfhydril group content. Taken together, these results indicate that e-liquid per se induces toxicity in Wistar rat testis, similar to e-liquid with nicotine, by disrupting oxidative balance and steroidogenesis.
Databáze:	OpenAIRE
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