Bioactivity screening of Aurantiochytrium sp. for antioxidant activities
| Autor: | Susana M. Bernardino, Maria Paulo, Carla Tecelão, Joaquina Pinheiro, Marco F.L. Lemos, Maria M. Gil, Teresa Baptista, Adriana Garcia, Joana Coutinho |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Antioxidant
DPPH medicine.medical_treatment Ocean Engineering antioxidant capacity Aquatic Science Oceanography 01 natural sciences chemistry.chemical_compound 0404 agricultural biotechnology Natural source medicine Food science Total phenolic content Aurantiochytrium sp Water Science and Technology Global and Planetary Change Chemistry 010401 analytical chemistry 04 agricultural and veterinary sciences 040401 food science 0104 chemical sciences Antioxidant capacity ORAC |
| Popis: | This study had the financial support by Research and Technological Development Incentive Program – Algavalue Project n.º 17680 – Valorização dos subprodutos do processo biotecnológico de produção de esqualeno e DHA pela microalga Aurantiochytrium sp. – and the strategic project UID/MAR/04292/2013 granted to MARE. Aurantiochytrium sp. is a heterotrophic microalgae that, in certain growth conditions, besides producing significant amounts of squalene, may potentially produce other bioactive compounds with high value and commercial interest such as docosahexaenoic acid (DHA), carotenoids, among others. Antioxidant compounds, naturally occurring in microalgae sources, have been identified as free radical or active oxygen scavengers, with beneficial effects on oxidation and oxidative damage inhibition in human body and foods. Several in vitro methodologies have been proposed to evaluate the antioxidant capacity, such as free radicals: 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2 -azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+), and reactive species including hydrogen peroxide (H2O2), singlet oxygen (O2), and superoxide anion (O2-) (Balboa et al., 2013). The main objective of the present study was to evaluate the antioxidant activity of aqueous and ethanolic extracts of Aurantiochytrium sp., by means of different methodologies: reducing and scavenging activity by total phenolics content (TPC, Singleton & Rossi, 1965), DPPH (Custódio et al., 2012), and oxygen radical absorbance capacity (ORAC, Dávalos et al., 2004). The obtained results indicated that the microalgae Aurantiochytrium sp. is rich in antioxidant compounds, by reducing activity through total phenolics and DPPH, as well as scavenging activity by ORAC. Moreover, it is evident the higher selectivity of ethanol as solvent for the extraction of antioxidants compounds. The potential of this microalgae biomass as a good, natural and sustainable source for use as a functional food ingredient is evident, representing an opportunity with additional benefits due to the antioxidant role in diseases prevention and treatment. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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