Cell Cycle Regulatory Proteins in the Liver in Murine Trypanosoma cruzi Infection
| Autor: | Stephen M. Factor, Herbert B. Tanowitz, Chris Albanese, Boumediene Bouzahzah, Stefka B. Petkova, Mahalia S. Desruisseaux, Mohan Krishnamachary, Murray Wittner, Louis M. Weiss, Fnu Nagajyothi, Richard G. Pestell, Alex W. Cohen, Shankar Mukherjee, Michael P. Lisanti, Linda A. Jelicks |
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| Rok vydání: | 2006 |
| Předmět: |
Cyclin E
Trypanosoma cruzi Cyclin D Cyclin A Cyclin B Cell Cycle Proteins Mice Inbred Strains Mice Cyclin D1 Cyclins Animals Chagas Disease RNA Messenger Molecular Biology Cell Proliferation Cyclin-Dependent Kinase Inhibitor Proteins Cyclin biology Cell Biology Cell cycle biology.organism_classification Virology Molecular biology Phenotype Liver biology.protein Tumor Suppressor Protein p53 Developmental Biology |
| Zdroj: | Cell Cycle. 5:2396-2400 |
| ISSN: | 1551-4005 1538-4101 |
| DOI: | 10.4161/cc.5.20.3380 |
| Popis: | The liver is an important target of Trypanosoma cruzi infection. Infection of CD-1 mice with T. cruzi (Brazil strain) resulted in parasitism of the liver, primarily in sinusoidal and Kupffer cells. Immunoblot analysis revealed activation of extra cellular signal-regulated kinase (ERK) during the acute and subacute period of infection, but p38 mitogen activated kinase (MAPK) and JNK were not activated. The activity of important cell cycle regulatory genes was also examined in the liver following infection. There was increased expression of cyclin D1, cyclin E and cyclin A as well as proliferating cell nuclear antigen (PCNA) at 45, 60 and 215 days post infection. In addition, the levels of the cyclin-dependent kinase inhibitors p27(KIP1), p21(WAF1) and the tumor suppressor p53 were increased in the livers obtained from infected mice. Quantitative PCR revealed increased abundance of mRNA for cyclins A, D1 and E. Interestingly, cyclin A and E are ordinarily not found in the adult liver. Thus infection caused a reversion to a fetal/neonatal phenotype. These data provide a molecular basis for cell proliferation in the liver following T. cruzi infection. |
| Databáze: | OpenAIRE |
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