A spike-timing-dependent plasticity rule for dendritic spines
| Autor: | Diana E. Mitchell, Roberto Araya, Soledad Miranda-Rottmann, Sabrina Tazerart |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
musculoskeletal diseases Dendritic spine Physiology Dendritic Spines Science Long-Term Potentiation Models Neurological General Physics and Astronomy Action Potentials 02 engineering and technology Plasticity Biology In Vitro Techniques Receptors N-Methyl-D-Aspartate General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Mice Postsynaptic potential Animals Calcium Signaling lcsh:Science Multidisciplinary Neuronal Plasticity Spike-timing-dependent plasticity Long-Term Synaptic Depression Pyramidal Cells Glutamate receptor Long-term potentiation General Chemistry 021001 nanoscience & nanotechnology musculoskeletal system Spine (zoology) Mice Inbred C57BL 030104 developmental biology Microscopy Fluorescence Multiphoton nervous system Excitatory postsynaptic potential lcsh:Q 0210 nano-technology Neuroscience |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-16 (2020) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | The structural organization of excitatory inputs supporting spike-timing-dependent plasticity (STDP) remains unknown. We performed a spine STDP protocol using two-photon (2P) glutamate uncaging (pre) paired with postsynaptic spikes (post) in layer 5 pyramidal neurons from juvenile mice. Here we report that pre-post pairings that trigger timing-dependent LTP (t-LTP) produce shrinkage of the activated spine neck and increase in synaptic strength; and post-pre pairings that trigger timing-dependent LTD (t-LTD) decrease synaptic strength without affecting spine shape. Furthermore, the induction of t-LTP with 2P glutamate uncaging in clustered spines (40 μm. These results indicate that synaptic cooperativity disrupts t-LTD and extends the temporal window for the induction of t-LTP, leading to STDP only encompassing LTP. The structural organization of excitatory inputs supporting spike-timing-dependent plasticity (STDP) in dendritic spines remains unknown. Using a spine STDP protocol, the authors uncover the STDP rules for single, clustered and distributed dendritic spines in the basal dendrites of layer 5 pyramidal neurons in juvenile mice. |
| Databáze: | OpenAIRE |
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