Toxic effects following phosgene exposure of human epithelial lung cells in vitro using a CULTEX® system
| Autor: | Marcel J. Alblas, Dorien Wijte, Jan P. Langenberg, D. Noort, Herman P.M. van Helden |
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| Rok vydání: | 2011 |
| Předmět: |
Atmosphere Exposure Chambers
Cell Survival Pharmacology Biology Toxicology chemistry.chemical_compound Cell Line Tumor Toxicity Tests medicine Humans Chemical Warfare Agents Phosgene Lung L-Lactate Dehydrogenase Interleukin-8 Epithelial Cells General Medicine Glutathione In vitro Oxidative Stress medicine.anatomical_structure chemistry Cell culture Latency stage Toxicity Animal studies Heme Oxygenase-1 |
| Zdroj: | Toxicology in Vitro. 25:2080-2087 |
| ISSN: | 0887-2333 |
| Popis: | The aim of the present study was to investigate toxic effects following phosgene exposure of human epithelial lung cells (A549) in vitro using a CULTEX® system. In particular, toxic effects regarding early biomarkers emerging during the latency period following exposure might be of great value for medical treatment. Cells cultured on semi-permeable membranes were directly exposed at the liquid-air interface to different concentrations of phosgene, or dry medical air. Cell membrane integrity (leakage of LDH), metabolic activity (reduction of Alamar Blue), oxidative damage (GSH, and HO-1, in cell lysates), and release of IL-8, were studied. For most of the above-mentioned biological end-point markers, significant changes could be assessed following a 20. min exposure to 1.0. ppm and 2.0. ppm phosgene. Moreover, except for IL-8, all biological marker profiles showed to be in line with results obtained by others in animal studies. The C×t value of 40. ppm. min appeared to be constant. The overall results suggest that at 4. h post-exposure a maximal level of toxicity was achieved. Our results demonstrate the suitability of a CULTEX® system to detect toxic effects induced by phosgene on human epithelial lung cells, which may contribute to the discovery of early biomarkers for new medical countermeasures. © 2011 Elsevier Ltd. |
| Databáze: | OpenAIRE |
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