Caught in the Act: Variation in plastid genome inverted repeat expansion within and between populations of Medicago minima
| Autor: | Tracey A. Ruhlman, Robert K. Jansen, In-Su Choi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Subfamily Inverted repeat homologous recombination 010603 evolutionary biology 01 natural sciences 03 medical and health sciences Monophyly lcsh:QH540-549.5 Clade Ecology Evolution Behavior and Systematics 030304 developmental biology Nature and Landscape Conservation 0303 health sciences Medicago minima Medicago plastid haplotype variation Ecology biology gene conversion Haplotype Fabaceae biology.organism_classification medicago inverted repeat expansion Evolutionary biology lcsh:Ecology alfalfa |
| Zdroj: | Ecology and Evolution, Vol 10, Iss 21, Pp 12129-12137 (2020) |
| ISSN: | 2045-7758 |
| Popis: | The inverted repeat (IR) lacking clade (IRLC) is a monophyletic group within the Papilionoideae subfamily of Fabaceae where plastid genomes (plastomes) do not contain the large IR typical of land plants. Recently, an IRLC legume, Medicago minima, was found to have regrown a ~9 kb IR that contained a number of canonical IR genes, and closely related M. lupulina contained an incomplete IR of ~425 bp. Complete plastomes were generated for seven additional species, putative members of the M. minima clade. Polymerase chain reaction was employed to investigate the presence of the IR across M. minima and M. lupulina including individuals of nine and eight Eurasian and North African accessions and 15 and 14 Texas populations, respectively. While no sequence similar to the ~9 kb IR was detected among the seven newly sequenced plastomes, all Eurasian and North African accessions of M. minima contained the IR. Variation in IR extent was detected within and between the Texas populations. Expansions of 13 bp and 11 bp occurred at the boundaries of both IR/small single‐copy regions, and populations had one or the other expansion, but not both. Expansion of the IR was not detected in the accessions from Eurasia and North Africa suggesting recent mutations yielded at least two additional plastid haplotypes in M. minima. |
| Databáze: | OpenAIRE |
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