Studies on Induction of Virus from Adenovirus and SV40 Hamster Tumors 1. Chemical and Physical Agents
| Autor: | G. A. Devers, V. M. Larson, B. J. Landau, Maurice R. Hilleman |
|---|---|
| Rok vydání: | 1966 |
| Předmět: |
Hydrocortisone
viruses Hamster Simian virus 40 Biology medicine.disease_cause General Biochemistry Genetics and Molecular Biology Virus Adenoviridae Mitomycins chemistry.chemical_compound Adrenocorticotropic Hormone Antigen Cricetinae medicine Animals Insulin Proflavine Mitomycin C Hydrogen Peroxide Neoplasms Experimental Virology Oxygen Radiation Effects Thyroxine chemistry Puromycin Cell culture Superinfection Dactinomycin Acridines |
| Zdroj: | Experimental Biology and Medicine. 122:1174-1182 |
| ISSN: | 1535-3699 1535-3702 |
| Popis: | SummaryCell culture lines of virus-free hamster tumors initiated by SV40 virus or by the Pinckney and Grider strains of adenovirus 7 were tested exhaustively by procedures which might be hoped to induce the virus or to increase its chance for detection. This included treatment with actinomycin D, mito-mycin C, puromycin, proflavine, hydrocorti-sone, ACTH, thyroxine, insulin, H2O2, increased O2, γ-radiation and copropagation with indicator cells. In no case was infectious virus recovered. Superinfection of SV40 hamster tumor with SV40 virus could not be established and hypothetical inapparent super-infection was not demonstrated following treatment with mitomycin C, proflavine or H2O2. No virus was detected in the cell cultures of any of 3 human tumors tested. The findings supported the concept that viral genetic materials in the animal tumors which are sufficient to code for “T” antigen may be insufficient to replicate the complete infectious virus. |
| Databáze: | OpenAIRE |
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