Mesenchymal Stem Cells Preserve Working Memory in the 3xTg-AD Mouse Model of Alzheimer’s Disease
| Autor: | Eva Syková, Pavla Jendelova, Magdalena Kulijewicz-Nawrot, Jose Julio Rodrigez-Arellano, Jiri Ruzicka |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Hippocampus lcsh:Chemistry Mice 0302 clinical medicine Lateral Ventricles Glutamate aspartate transporter lcsh:QH301-705.5 Spectroscopy biology Alzheimer’s disease mesenchymal stem cells working memory Aβ*56 neurogenesis Neurogenesis General Medicine Anatomy Computer Science Applications Memory Short-Term medicine.anatomical_structure Stem cell medicine.medical_specialty Subventricular zone Mice Transgenic Mesenchymal Stem Cell Transplantation Article Catalysis Inorganic Chemistry 03 medical and health sciences Alzheimer Disease Glutamate-Ammonia Ligase Internal medicine medicine Animals Humans Physical and Theoretical Chemistry Maze Learning Molecular Biology Cell Proliferation Amyloid beta-Peptides Organic Chemistry Mesenchymal stem cell Entorhinal cortex Transplantation Disease Models Animal 030104 developmental biology Endocrinology lcsh:Biology (General) lcsh:QD1-999 biology.protein 030217 neurology & neurosurgery |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 17; Issue 2; Pages: 152 International Journal of Molecular Sciences, Vol 17, Iss 2, p 152 (2016) |
| ISSN: | 1422-0067 |
| Popis: | The transplantation of stem cells may have a therapeutic effect on the pathogenesis and progression of neurodegenerative disorders. In the present study, we transplanted human mesenchymal stem cells (MSCs) into the lateral ventricle of a triple transgenic mouse model of Alzheimer´s disease (3xTg-AD) at the age of eight months. We evaluated spatial reference and working memory after MSC treatment and the possible underlying mechanisms, such as the influence of transplanted MSCs on neurogenesis in the subventricular zone (SVZ) and the expression levels of a 56 kDa oligomer of amyloid β (Aβ*56), glutamine synthetase (GS) and glutamate transporters (Glutamate aspartate transporter (GLAST) and Glutamate transporter-1 (GLT-1)) in the entorhinal and prefrontal cortices and the hippocampus. At 14 months of age we observed the preservation of working memory in MSC-treated 3xTg-AD mice, suggesting that such preservation might be due to the protective effect of MSCs on GS levels and the considerable downregulation of Aβ*56 levels in the entorhinal cortex. These changes were observed six months after transplantation, accompanied by clusters of proliferating cells in the SVZ. Since the grafted cells did not survive for the whole experimental period, it is likely that the observed effects could have been transiently more pronounced at earlier time points than at six months after cell application. |
| Databáze: | OpenAIRE |
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