Proteomic Analysis of Morphologically Changed Tissues after Prolonged Dexamethasone Treatment

Autor: Anas M. Abdel Rahman, Minnie Jacob, Abeer Malkawi, Goran Matic, Ayodele Alaiya, Majed Dasouki, Zakia Shinwari, Afshan Masood, Hicham Benabdelkamel, Falah Almuhanna
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Male
Proteomics
Proteome
Pharmacology
Nucleic acid metabolism
LC-MS/M
proteomic expression
lcsh:Chemistry
chemistry.chemical_compound
Tandem Mass Spectrometry
Gene Regulatory Networks
rat tissues
lcsh:QH301-705.5
Spectroscopy
glucocorticoid side effects
Kidney
General Medicine
Computer Science Applications
medicine.anatomical_structure
Organ Specificity
medicine.symptom
medicine.drug
Signal Transduction
Connective tissue
dexamethasone
Biology
Kidney cysts
Catalysis
Article
Inorganic Chemistry
Atrophy
label-free proteomics
network pathway
medicine
Animals
Physical and Theoretical Chemistry
Molecular Biology
Dexamethasone
Gene Expression Profiling
Organic Chemistry
Computational Biology
Metabolism
medicine.disease
Rats
Gene Ontology
chemistry
Gene Expression Regulation
lcsh:Biology (General)
lcsh:QD1-999
Chromatography
Liquid
Zdroj: International Journal of Molecular Sciences
Volume 20
Issue 13
International Journal of Molecular Sciences, Vol 20, Iss 13, p 3122 (2019)
ISSN: 1422-0067
DOI: 10.3390/ijms20133122
Popis: Prolonged dexamethasone (Dex) administration leads to serious adverse and decrease brain and heart size, muscular atrophy, hemorrhagic liver, and presence of kidney cysts. Herein, we used an untargeted proteomic approach using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for simultaneous identification of changes in proteomes of the major organs in Sprague&ndash
Dawley (SD rats post Dex treatment. The comparative and quantitative proteomic analysis of the brain, heart, muscle, liver, and kidney tissues revealed differential expression of proteins (n = 190, 193, 39, 230, and 53, respectively) between Dex-treated and control rats. Functional network analysis using ingenuity pathway analysis (IPA revealed significant differences in regulation of metabolic pathways within the morphologically changed organs that related to: (i) brain&mdash
cell morphology, nervous system development, and function and neurological disease
(ii) heart&mdash
cellular development, cellular function and maintenance, connective tissue development and function
(iii) skeletal muscle&mdash
nucleic acid metabolism, and small molecule biochemical pathways
(iv) liver&mdash
lipid metabolism, small molecular biochemistry, and nucleic acid metabolism
and (v) kidney&mdash
drug metabolism, organism injury and abnormalities, and renal damage. Our study provides a comprehensive description of the organ-specific proteomic profilesand differentially altered biochemical pathways, after prolonged Dex treatement to understand the molecular basis for development of side effects.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje