Presence of activation-related m-RNA for EBV and CMV in the bone marrow of patients with myelodysplastic syndromes
| Autor: | Azra Raza, Parameswaran Venugopal, Sairah Alvi, Krishnan Allampallam, Khwaja Aftab Rashid, Bruce Dangerfield, Daniel Zeitler, Evans K. Afenya, Suneel D. Mundle, Jonathan D. Cartlidge, Vilasini Shetty |
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| Rok vydání: | 2001 |
| Předmět: |
Herpesvirus 4
Human Cancer Research Pathology medicine.medical_specialty Stromal cell Cytomegalovirus medicine.disease_cause Virus Herpesviridae Immediate early protein Bone Marrow Betaherpesvirinae hemic and lymphatic diseases medicine Humans RNA Messenger biology Reverse Transcriptase Polymerase Chain Reaction Myelodysplastic syndromes Fetal Blood medicine.disease biology.organism_classification Epstein–Barr virus medicine.anatomical_structure Oncology Myelodysplastic Syndromes Immunology Virus Activation Bone marrow Stromal Cells |
| Zdroj: | Cancer Letters. 164:197-205 |
| ISSN: | 0304-3835 |
| Popis: | The bone marrow (BM) in myelodysplastic syndromes (MDS) undergoes pathobiological changes that mimic an inflammatory process, and hence, an infectious etiology was suspected in these disorders. In the present report, we examined the bone marrow mononuclear cells (BMMNC) of 19 MDS patients and seven normal donors for the expression of one latency-related (Latency membrane protein 1 (LMP-1) and immediate early protein (IEP)) and one activation-related (BZLF and DNA-Pol) m-RNA each for two herpes viruses, Epstein-Barr virus (EBV) and cytomegalovirus (CMV), respectively. Reverse transcriptase polymerase chain reaction was used for this purpose. The latency-related transcripts (EBV-LMP-1 and CMV-IEP) were present in all the MDS and normal specimens. Intriguingly, 10/19 MDS specimens ( approximately 53%) and 2/7 normal donors ( approximately 28%) were positive for active EBV-BZLF (P=0.0067), while 2/19 MDS specimens ( approximately 11%) with 1/7 normal ( approximately 14%) showed active CMV-DNA-Pol (P=0.1588). Later, from another set of MDS patients (n=7) and normal donors (n=4), BM stromal cultures were established, which, at a 75% confluency, were overlaid with cord blood mononuclear cells (CBMNC). IEP was detectable in the CBMNC before and after co-incubation with MDS, as well as normal stroma. So, it was also present both in MDS and normal stromal cells. The other three were absent both in MDS and normal stromal layers. In CBMNC though, active EBV-BZLF and CMV-DNA-Pol m-RNA were detectable in one of seven MDS co-cultures each, albeit from different patients. None of the normal co-cultures showed active virus, either in stroma or CBMNC. Thus, the present report demonstrates, for the first time, the presence of active herpes viruses in the BMMNC of MDS patients and reveals the ability of the MDS stroma to support the viral activation. |
| Databáze: | OpenAIRE |
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