Decreased expression of interleukin 13 in human lung emphysema
| Autor: | Bruno Crestani, Véronique Leçon-Malas, G Durand, Michel Fournier, Michel Aubier, Anne Boutten, S Laribe, Monique Dehoux, Marcel Bonay, Guy Lesèche, Y Castier |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Pulmonary and Respiratory Medicine
Adult Male medicine.medical_specialty Pathology DNA Complementary Chronic Obstructive Pulmonary Disease Vital Capacity Gastroenterology Human lung Interferon-gamma Interferon Internal medicine Forced Expiratory Volume Gene expression medicine Humans skin and connective tissue diseases Aged Messenger RNA Lung Interleukin-13 business.industry Respiratory disease Interleukin respiratory system Middle Aged medicine.disease respiratory tract diseases medicine.anatomical_structure Pulmonary Emphysema Interleukin 13 RNA Female sense organs business medicine.drug |
| Zdroj: | Thorax. 59(10) |
| ISSN: | 0040-6376 |
| Popis: | Background: The overexpression of interferon (IFN)γ or interleukin (IL)-13 in the adult murine lung induces the development of changes that mirror human lung emphysema. Methods: IL-13 and IFNγ expression was determined in lung samples from five groups of patients: severe emphysema without α 1 -antitrypsin deficiency (SE+, n = 10); severe emphysema with α 1 -antitrypsin deficiency (SE−, n = 5); mild localised emphysema (ME, n = 8); non-emphysema smokers (NE-S, n = 9), and non-emphysema non-smokers (NE-NS, n = 11). Lung IL-13 and IFNγ mRNA were analysed by RT-PCR. Lung concentrations of IL-13 protein were assessed by ELISA. Results: The expression of IFNγ mRNA was similar in patients with or without emphysema. IL-13 mRNA was markedly decreased in the SE+ group compared with the SE− (p = 0.04), ME (p = 0.02), and non-emphysema groups (p = 0.01). IL-13 mRNA correlated with forced expiratory volume in 1 second ( r = 0.5, p = 0.04) and arterial oxygen tension ( r = 0.45, p = 0.03) in emphysema patients. In contrast to the non-emphysematous lung, IL-13 protein was below the detection limit of the assay in most emphysematous lung homogenates. Conclusion: The lung IL-13 content is reduced in patients with severe emphysema without α 1 -antitrypsin deficiency. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|