T-cell-dependent modulation of the polyclonal B-lymphocyte responses in normal spleen cell cultures stimulated by lipopolysaccharide
| Autor: | Luciana Forni, Mariana Björklund, Antonio Coutinho |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Lipopolysaccharides Male Lipopolysaccharide T-Lymphocytes Lymphocyte T cell Mice Inbred Strains Biology Lymphocyte Activation Mice chemistry.chemical_compound Cell–cell interaction Precursor cell Splenocyte medicine Animals Immunologic Surveillance Cells Cultured General Environmental Science B-Lymphocytes Immunity Cellular General Medicine T lymphocyte Molecular biology medicine.anatomical_structure chemistry Cell culture Immunoglobulin G Antigens Surface Immunology General Earth and Planetary Sciences Female Spleen |
| Zdroj: | Annales de l'Institut Pasteur / Immunologie. 138:181-199 |
| ISSN: | 0769-2625 |
| DOI: | 10.1016/s0769-2625(87)80070-3 |
| Popis: | Summary The in vitro polyclonal B-cell proliferative and plaque-forming cell (PFC) responses to the T-independent (TI) mitogen lipopolysaccharide (LPS) are increased by the addition of normal syngeneic splenic T cells. Normal irradiated Lyt-2 − T cells also alter the IgG subclass distribution from the typical predominance of IgG3 and IgG2b PFC to the appearance of IgG1, IgG2a and IgA PFC in T-cell-depleted spleen cell (SC) cultures. Furthermore, secondary LPS blast cultures yield increased PFC responses when co-cultured which syngeneic fresh normal T cells which, even in the absence of mitogen, induce PFC responses in such activated B cells. As LPS blasts induce normal syngeneic T cells to proliferate and significant numbers of L3T4 + blast cells are found in LPS-stimulated normal spleen cell cultures, we conclude that T cells actively participate in the regulation of these responses. The significance of these findings for the regulation of TI responses in vivo by «autoreactive» T cells is considered. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|