Differences of two Borrelia burgdorferi strains in complement activation and serum resistance
| Autor: | Georg Acker, Volker Brade, Ingrid Kleber |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
biology
Immunology Cell Hematology Complement C9 biology.organism_classification Antibodies Bacterial Incubation period Microbiology Complement system medicine.anatomical_structure Borrelia burgdorferi Group Species Specificity Immunoglobulin G biology.protein medicine Humans Immunology and Allergy Bleb (cell biology) Antibody Borrelia burgdorferi Complement Activation Incubation Bacteria |
| Zdroj: | Immunobiology. 185:453-465 |
| ISSN: | 0171-2985 |
| DOI: | 10.1016/s0171-2985(11)80087-2 |
| Popis: | Complement activation and serum resistance of the Borrelia burgdorferi strains B31 (American strain) and PKo (European strain) were compared. In 25% (v/v) normal human serum (NHS) free of B. burgdorferi-specific antibodies the cells of the PKo strain were high activators of complement as indicated by rapid and strong C9 consumption, by deposition of up to 336763 C9 molecules per cell and by the formation of the terminal complement complex on the cell surface. By comparison, complement activation by the B31 strain was low with 5.4-fold less C9 deposited per cell. The addition of B. burgdorferi-specific antibodies to NHS either as purified IgG or heat-inactivated patient sera, had no influence on the results with both strains. After an incubation period of 2h at 37 degrees C in 25% (v/v) NHS most cells of the PKo strain had lost their viability as indicated by cell immobilization and failure to multiply in subcultures. In addition, extensive cell fragmentation and bleb formation were observed in the electron microscope. In contrast, the B31 strain remained alive and morphologically intact after the same incubation with NHS. We conclude from our results that complement activation and serum resistance are properties which differ considerably between isolated strains of B. burgdorferi. |
| Databáze: | OpenAIRE |
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