Hypothalamic Proline-Rich Polypeptide Enhances Bone Marrow Colony-Forming Cell Proliferation and Stromal Progenitor Cell Differentiation
| Autor: | T. K. Davtyan, N. A. Galoyan, E. J. Rybalkina, K. B. Bezirganyan, A. A. Galoyan, A. V. Revishchin, Leonid I. Korochkin, E. I. Zaraiski, I.N. Saburina, Galina Pavlova |
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| Rok vydání: | 2008 |
| Předmět: |
Stromal cell
Molecular Sequence Data Hypothalamus Biomedical Engineering lcsh:Medicine Bone Marrow Cells Peptide Antigens CD medicine Animals Humans Amino Acid Sequence Rats Wistar Progenitor cell Proline rich Cells Cultured Cell Proliferation chemistry.chemical_classification Transplantation Chemistry Cell growth Stem Cells Neuropeptides lcsh:R Cell Differentiation Cell Biology Rats Cell biology Arginine Vasopressin Endothelial stem cell medicine.anatomical_structure Cattle Bone marrow Myelopoiesis Stromal Cells Peptides Antimicrobial Cationic Peptides |
| Zdroj: | ResearcherID Cell Transplantation, Vol 17 (2008) |
| ISSN: | 1555-3892 0963-6897 |
| DOI: | 10.3727/096368908786991579 |
| Popis: | The AGAPEPAEPAQPGVY proline-rich peptide (PRP-1) was isolated from neurosecretory granules of the bovine neurohypophysis; it is produced by N. supraopticus and N. paraventricularis. It has been shown that PRP-1 has many potentially beneficial biological effects, including immunoregulatory, hematopoietic, antimicrobial, and antineurodegenerative properties. Here we showed that PRP increased colony-forming cell (CFC) proliferation in rat bone marrow (BM) cells in vivo. In PRP-treated rat BM, the CFU number at day 7 and day 14 was considerably increased in comparison with untreated rat BM and no difference was found at day 21 and day 28. The related peptide [arg8]vasopressin did not reveal CFC proliferation. PRP failed to farther increase CFC proliferation in vitro in BM obtained from PRP-treated or untreated rats. After 3–4 days of human BM stromal cell cultivation in the presence of 2–20 μg/ml PRP the appearance of cells expressing CD15, CD10, CD11a, CD11b, CD3, CD4, and CD16 surface antigens did not differ from the untreated cells. PRP increased the appearance of CD14-positive cells upon 3–4-day incubation with both adult and fetal BM stromal cells. Our results suggest a previously undescribed role for the hypothalamic peptide within neurosecretory hypothalamus–bone marrow humoral axis, because PRP enhances BM colony-forming cell proliferation and stromal cell differentiation. |
| Databáze: | OpenAIRE |
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