Human Kaposi's Sarcoma Cell-Mediated Tumorigenesis in Human Immunodeficiency Type 1 Tat-Expressing Transgenic Mice
| Autor: | Gist Farr, Om Prakash, You E. He, Roy Coleman, Javed Gill, Zhenya Tang, Manzoor S. Ali, Felipe Samaniego |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Male
Genetically modified mouse Cancer Research Genes Viral Neutrophils medicine.medical_treatment Basic fibroblast growth factor Gene Expression Mice Nude Mice Transgenic Mice chemistry.chemical_compound Tumor Cells Cultured medicine Animals Humans Tissue Distribution RNA Messenger Interleukin 8 Interleukin 6 Sarcoma Kaposi Kaposi's sarcoma biology NF-kappa B Metalloendopeptidases Neoplasms Experimental NFKB1 medicine.disease Virology Molecular biology Cytokine Neutrophil Infiltration Oncology chemistry Gene Products tat HIV-1 biology.protein tat Gene Products Human Immunodeficiency Virus Tumor necrosis factor alpha Extravasation of Diagnostic and Therapeutic Materials |
| Zdroj: | Journal of the National Cancer Institute. 92:721-728 |
| ISSN: | 1460-2105 |
| DOI: | 10.1093/jnci/92.9.721 |
| Popis: | BACKGROUND The human immunodeficiency virus type 1 (HIV-1) transactivator (Tat) protein has been linked to the development and course of Kaposi's sarcoma (KS) associated with acquired immunodeficiency disease syndrome (AIDS-KS). Tat is an 86-101 amino-acid protein encoded by two exons. To evaluate the growth-promoting effects of Tat in AIDS-KS in vivo, we developed transgenic mice expressing the one-exon-encoded 72 amino-acid protein (Tat(72)) and the two-exon-encoded 86 amino-acid protein (Tat(86)). METHODS Human KS SLK cells were injected subcutaneously into CD4(+) T-cell-depleted male mice, and the tumors that formed after 3-4 weeks were recovered and analyzed for the expression of Tat protein(s), different cytokine messenger RNAs (mRNAs), and matrix metalloproteinases (MMPs). All statistical tests were two-sided. RESULTS The average tumor weight was maximum in Tat(86) mice ( approximately 600 mg) compared with Tat(72) ( approximately 200 mg) and nontransgenic ( approximately 100 mg) mice (P |
| Databáze: | OpenAIRE |
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