Effect of mercury substitution of DNA on its susceptibility to cleavage by restriction endonucleases
| Autor: | Nilima Sarkar, Gaspar Banfalvi |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
endocrine system
viruses DNA polymerase II Molecular Sequence Data Cleavage (embryo) Restriction fragment chemistry.chemical_compound Restriction map Genetics Escherichia coli heterocyclic compounds A-DNA Molecular Biology biology Base Sequence food and beverages Cell Biology General Medicine DNA DNA Restriction Enzymes Mercury Molecular biology Restriction enzyme Biochemistry chemistry biology.protein Electrophoresis Polyacrylamide Gel DNA polymerase I |
| Zdroj: | DNA and cell biology. 14(5) |
| ISSN: | 1044-5498 |
| Popis: | Mercurated DNA was synthesized in vitro by substituting Hg-dCTP or Hg-dUMP for dCTP in one strand of M13mp8DNA in a DNA polymerase I reaction. Restriction enzymes, including Sma I, Pst I, Bam HI, Hind III, and Hinc II, were completely inactive when their recognition sites were fully substituted with Hg-dCMP, while Hg-dUMP containing DNA was hydroxlyzed to some extent. Under conditions favoring star activities, or when DNA was substituted with a low level of mercury-nucleotide, DNA was cleaved by restriction enzymes. Susceptibility to degrading and synthesizing enzymes and insensitivity to restriction endonucleases of fully mercurated DNA makes mercuration an attractive molecular "tag" for in vitro manipulation and selective isolation of Hg-DNA. |
| Databáze: | OpenAIRE |
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