Neurodegeneration-related beta-amyloid as autocatabolism-attenuator in a micro-in vivo system
| Autor: | Zita Galik-Olah, Evelin Balazs, Zsolt Datki, Janos Kalman, Zsolt Bozsó, Bence Galik |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
S-Aβ42 scrambled isoform of Aβ Attenuator (genetics) Peptide Beta-amyloid BisANS 4 4′-dianilino-1 1′-binaphthyl-5 5′-disulfonic acid dipotassium salt BisANS (PubChem CID: 16213473) 0302 clinical medicine D20 Day 20 Invertebrate chemistry.chemical_classification 0303 health sciences Chemistry General Neuroscience Neurodegeneration FROSi FROS index AVOs acidic vesicular organelles PI propidium-iodide Cell biology Amino acid D0 Day 0 Organ shrinkage NaOH (PubChem CID: 14798) ConA Concanamycin A AO acridine orange Short Communication Propidium-iodide (PubChem CID: 104981) FROS functionally reversible organ shrinkage SEM standard error of the mean lcsh:RC321-571 Aβ beta-amyloid 03 medical and health sciences Neutral red (PubChem CID: 11105) In vivo Acridine orange (PubChem CID: 62344) Autocatabolism medicine Concanamycin A (PubChem CID: 6438151) Enhancer lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry 030304 developmental biology Size reduction NFI% percentage of normalized fluorescence intensity Metabolism Organ Size medicine.disease D25 Day 25 030104 developmental biology 030217 neurology & neurosurgery Bdelloid rotifer |
| Zdroj: | bioRxiv IBRO Reports, Vol 9, Iss, Pp 319-323 (2020) IBRO Reports |
| DOI: | 10.1101/2020.04.27.063115 |
| Popis: | Highlights • Bdelloids are adaptive models for studying aggregate-metabolism interactions. • Starvation causes reversible organ shrinkage in bdelloids. • The organ shrinkage is in connection with autocatabolic processes. • Beta-amyloid attenuates the starvation-induced germovitellaria shrinkage. • Human-type amyloid-aggregates are metabolism-regulators in two bdelloid species. Investigation of human neurodegeneration-related aggregates of beta-amyloid 1–42 (Aβ42) on bdelloid rotifers is a novel interdisciplinary approach in life sciences. We reapplied an organ size-based in vivo monitoring system, exploring the autocatabolism-related alterations evoked by Aβ42, in a glucose-supplemented starvation model. The experientially easy-to-follow size reduction of the bilateral reproductive organ (germovitellaria) in fasted rotifers was rescued by Aβ42, serving as a nutrient source- and peptide sequence-specific attenuator of the organ shrinkage phase and enhancer of the regenerative one including egg reproduction. Recovery of the germovitellaria was significant in comparison with the greatly shrunken form. In contrast to the well-known neurotoxic Aβ42 (except the bdelloids) with specific regulatory roles, the artificially designed scrambled version (random order of amino acids) was inefficient in autocatabolism attenuation, behaving as negative control. This native Aβ42-related modulation of the ‘functionally reversible organ shrinkage’ can be a potential experiential and supramolecular marker of autocatabolism in vivo. |
| Databáze: | OpenAIRE |
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