In vitro cell cytotoxicity profile and morphological response to polyoxometalate-stabilised gold nanoparticles
| Autor: | María Moros, Isabel Maicas Gabas, Jesús M. de la Fuente, Scott G. Mitchell, Grazyna Stepien |
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| Přispěvatelé: | Fundación General CSIC, European Research Council |
| Rok vydání: | 2016 |
| Předmět: |
animal structures
Cell cycle checkpoint Stereochemistry Chemistry Nanoparticle 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Catalysis In vitro 3. Good health 0104 chemical sciences molecular polyoxometalates Colloidal gold Cell culture Cytoplasm Materials Chemistry Biophysics Vero cell sense organs Viability assay 0210 nano-technology |
| Zdroj: | Zaguán. Repositorio Digital de la Universidad de Zaragoza instname BASE-Bielefeld Academic Search Engine Digital.CSIC. Repositorio Institucional del CSIC New journal of chemistry (1987) 40 (2016): 1039–1047. doi:10.1039/c5nj02775f info:cnr-pdr/source/autori:Gabas I.M.; Stepien G.; Moros M.; Mitchell S.G.; De La Fuente J.M./titolo:In vitro cell cytotoxicity profile and morphological response to polyoxometalate-stabilised gold nanoparticles/doi:10.1039%2Fc5nj02775f/rivista:New journal of chemistry (1987)/anno:2016/pagina_da:1039/pagina_a:1047/intervallo_pagine:1039–1047/volume:40 |
| ISSN: | 1369-9261 1144-0546 |
| DOI: | 10.1039/c5nj02775f |
| Popis: | The size and redox properties of molecular polyoxometalates (POMs) make them extremely relevant for bioapplications: from disrupting tumour growth and enzyme inhibition, to DNA-intercalating agents and antimicrobial applications. Their unique ability to reversibly dominate and receive electrons, coupled with their high anionic charge, also makes them suitable for the preparation of zero-valent state metal nanoparticles (NPs) from molecular precursors. Polyoxometalate-stabilised nanoparticles (NPs@POM) are therefore an ideal delivery vehicle for bioactive POMs. Here we show how POM-stabilised gold NPs (AuNPs@POM) are massively internalised into Vero (kidney epithelial) and B16 (skin melanoma) cell lines with variable cytotoxic effects. Cell viability assays and quantification of cytoplasmic membrane composition revealed that the Vero cell line was unaltered by the internalisation of these hybrid particles; while their internalisation in B16 tumour cells produced mild cytotoxic effects and an antiproliferative cell cycle arrest in the G0/G1 and G2/M phases. The observed perturbation of the tumour cell line combined with the high degree of internalisation means that these (or similar) NPs@POM could serve as candidates for a range of bioapplications in diagnostics or therapy. Financial support by the Fundación General CSIC (Programa ComFuturo) and the ERC-Starting Grant 239931-NANOPUZZLE is acknowledged. |
| Databáze: | OpenAIRE |
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