Physical linkage of the SLG and SRK genes at the self-incompatibility locus of Brassica oleracea
| Autor: | June B. Nasrallah, Douglas C. Boyes |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Genetic Linkage
Locus (genetics) Brassica Genes Plant chemistry.chemical_compound Genotype Genetics Deoxyribonucleases Type II Site-Specific Molecular Biology Gene Crosses Genetic Glycoproteins Plant Proteins Gel electrophoresis biology Homozygote food and beverages Nucleic Acid Hybridization biology.organism_classification Phenotype Electrophoresis Gel Pulsed-Field chemistry Haplotypes Brassica oleracea Pollen Restriction fragment length polymorphism DNA Probes Protein Kinases DNA |
| Zdroj: | Moleculargeneral genetics : MGG. 236(2-3) |
| ISSN: | 0026-8925 |
| Popis: | In Brassica oleracea, the pollen-stigma interaction of self-incompatibility is controlled by a single genetically defined locus designated S. Molecular studies have identified two genes that are tightly linked to the classically defined S locus: The S-Locus Glycoprotein (SLG) gene and the S-Receptor Kinase (SRK) gene. In previous RFLP linkage analyses with probes specific for SLG and SRK, we were unable to identify any recombination events between SLG, SRK, and self-incompatibility phenotype. In this paper, we use pulsed-field gel electrophoresis (PFGE) in conjunction with DNA blot analysis to characterize the S-locus region from two highly divergent self-incompatibility genotypes, S2 and S6. We establish the physical linkage of SLG and SRK in each genotype, and demonstrate that the two genes are separated by a maximum distance of 220 kb in the S6 genotype and 350 kb in the S2 genotype. Furthermore, a comparison of the data from the two genotypes reveals that a high level of polymorphism exists across the entire S-locus region. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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