Microbial Biomarkers in Patients with Nonresponsive Celiac Disease
| Autor: | Tuire Ilus, Päivi Saavalainen, Katri Kaukinen, Merja Ashorn, Kalle Kurppa, Sari Iltanen, Heini Huhtala, Liisa Viitasalo, Markku Mäki, Sara Ashorn |
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| Přispěvatelé: | Immunomics, Immunobiology Research Program, Department of Medical and Clinical Genetics, Clinicum, Research Programs Unit, University of Helsinki, Medicum |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Physiology Biopsy Microbial biomarkers Disease Inflammatory bowel disease Gastroenterology Endoscopy Gastrointestinal NOD2/CARD15 GENOTYPE 0302 clinical medicine Bacteroides Treatment Failure Bacteroides caccae Correlation of Data Nonresponsive celiac disease Finland biology Microbiota Middle Aged Antibodies Bacterial Immunohistochemistry CROHNS-DISEASE 3. Good health Titer 030211 gastroenterology & hepatology Female PERSISTENT SYMPTOMS Antibody medicine.medical_specialty ANTI-SACCHAROMYCES-CEREVISIAE Duodenum IBD Saccharomyces cerevisiae Pseudomonas fluorescens 03 medical and health sciences Diet Gluten-Free Internal medicine medicine Humans SEROLOGICAL RESPONSES In patient Serologic Tests GLUTEN-FREE DIET business.industry Pseudomonas fluorescence ASCA BACTEROIDES CACCAE Hepatology medicine.disease Gastrointestinal Microbiome Celiac Disease 030104 developmental biology 3121 General medicine internal medicine and other clinical medicine ANTIBODIES biology.protein Dysbiosis 3111 Biomedicine business INFLAMMATORY-BOWEL-DISEASE |
| Popis: | Background and AimsIn nonresponsive celiac disease (NRCD), the symptoms and duodenal damage persist despite a gluten-free diet. Celiac disease patients with persistent symptoms are found to have a dysbiotic microbiota. We thus hypothesized that increased seroreactivity to the serum gluten-sensitive microbial antibodies Saccharomyces cerevisiae (ASCA), Pseudomonas fluorescens-associated sequence (I2), and Bacteroides caccae TonB-linked outer membrane protein (OmpW) is associated with NRCD.MethodsASCA, I2 and OmpW were measured in 20 seronegative CD patients with persistent villous damage despite strict dietary treatment (NRCD group). Fifty-eight responsive patients served as CD controls (55 on gluten-free treatment) and 80 blood donors as non-CD controls.ResultsAt least one microbial marker was positive in 80% of NRCD patients, in 97% of untreated CD and 87% of treated CD patients, and in 44% of controls. NRCD patients had the highest frequency of ASCA positivity (65% vs 52, 20, and 0%, respectively) and also significantly higher ASCA IgA (median 14.5 U/ml) and IgG (32.5 U/ml) titers than treated CD patients (7.0 U/ml, 13.0 U/ml) and non-CD controls (4.5 U/ml, 5.8 U/ml). The frequencies of I2 and OmpW were lower in NRCD than in untreated CD (65% and 45% vs 86% and 59%, respectively), and I2 titers were higher in NRCD (median absorbance 0.76) and untreated (1.0) and treated (0.83) CD than controls (0.32). OmpW was elevated in untreated (1.1) and treated (0.94) CD patients compared with controls (0.79).ConclusionsSeropositivity and high titers of ASCA are associated with NRCD and might serve as an additional follow-up tool in CD. |
| Databáze: | OpenAIRE |
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