Optical molecular imaging of lymph nodes using a targeted vascular contrast agent
| Autor: | Kai Licha, Wolfhard Semmler, Dietger Stibenz, Sonja Emig-Vollmer, Rudolf Tauber, Diethard Petzelt, Michael Hasbach, Niels Debus, Sabine Sydow, Birte Hofmann, Michael Schirner, Christoph Bührer, Bernd Ebert |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Pathology medicine.medical_specialty Spectrophotometry Infrared Metabolic Clearance Rate High endothelial venules Biomedical Engineering Contrast Media Mice Nude Biomaterials Mice Drug Delivery Systems medicine Animals Tissue Distribution Lymphocyte homing receptor Lymph node Fluorescent Dyes Lymphatic Vessels Chemistry Antibodies Monoclonal Membrane Proteins Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials medicine.anatomical_structure Lymphatic system Microscopy Fluorescence Antigens Surface Female Lymph Nodes Lymph Peripheral lymph Preclinical imaging |
| Zdroj: | Journal of Biomedical Optics. 10:041205 |
| ISSN: | 1083-3668 |
| DOI: | 10.1117/1.2007967 |
| Popis: | We develop a highly specific antibody-dye conjugate for optical imaging of peripheral lymph nodes. The contrast agent consists of the monoclonal antibody recognizing endothelial ligands for the lymphocyte homing receptor L-selectin, MECA-79, and a near-infrared (near-IR) fluorescent indotricarbocyanine dye. The targeting and biodistribution behavior of MECA-79 is studied after radio-iodination and intravenous injection into mice demonstrating specific uptake in lymph nodes and accumulation in high endothelial venules (HEV). After conjugation of MECA-79 with indotricarbocyanine dye, the fluorescence imaging properties of the MECA-79 dye conjugate are examined by intravenous injection in nude mice and laser-induced fluorescence whole-body imaging in vivo. The MECA-79 antibody-dye conjugate accumulates in peripheral lymph nodes, whereas an isotype antibody-dye conjugate does not. Specific lymph node near-IR fluorescent signals become detectable within minutes after injection, and stable imaging persists for more than 24 h. The results demonstrate that vascular targeting of endothelial expression of glyocproteins is feasible to visualize the accumulation of near-IR fluorescent MECA-79 in lymph nodes, making this technology potentially useful to characterize processes of inflammation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|