Isolated Hemoperfused Slaughterhouse Livers as a Valid Model to Study Hepatotoxicity
| Autor: | David A. Groneberg, Christian Grosse-Siestrup, Stefan Nagel, Axel Fischer, Christian Witt, Jeanette Pfeffer, Volker Unger |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Pathology
medicine.medical_specialty Diclofenac Blood transfusion Swine medicine.medical_treatment Analgesic Pharmacology Toxicology 030226 pharmacology & pharmacy Pathology and Forensic Medicine Blood Transfusion Autologous 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals Molecular Biology Dialysis Creatinine business.industry Anti-Inflammatory Agents Non-Steroidal Organ Preservation Cell Biology Oxygenation Hemoperfusion Disease Models Animal Liver chemistry 030220 oncology & carcinogenesis Toxicity Chemical and Drug Induced Liver Injury business medicine.drug |
| Zdroj: | Toxicologic Pathology. 30:749-754 |
| ISSN: | 1533-1601 0192-6233 |
| DOI: | 10.1080/01926230290166841 |
| Popis: | Different models of isolated and perfused livers and precision cut liver slices have been developed for studies on liver toxicology the past years. As most of these models were limited by nonphysiologi c settings, a new model of normothermic hemoperfused isolated porcine slaughterhous e livers to examine hepatotoxicity was established encompassing the advantages of slaughterhouse organs to reduce animal experiments and autologous blood as an optimal perfusate. As model compound, the analgesic substance diclofenac was used and the effects of this drug on organ function parameters were compared to an untreated control group. Using an amount of 2,000 ml, the organs were perfused over 180 minutes, metabolically controlled via a dialysis and oxygenation system and various hematological and hepatic parameters were examined. In contrast to the untreated control organs, significant differences were found in the diclofenac group for parameters such as lactate, creatinine, ALT, bicarbonate, or bile flow. In summary, the presently established model of isolated hemoperfused slaughterhouse livers displays a useful new approach to assess hepatotoxicity of different substances on the organ level. As a major economic advantage in comparison to setups using laboratory animals, the new model can be run with blood and organs obtained from slaughterhouse animals. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|