The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans
| Autor: | Joshua Wt Anderson, Menachem J. Gunzburg, Timothy P. Hughes, Debashish Ray, Gregory M. Davis, Uri Seroussi, Sean P Shrubsole, Monica Z. Wu, Julie M. Claycomb, Peter R. Boag, Quaid Morris, Zhiping Weng, Jacqueline A. Wilce, Julia A. Sobotka, Shikui Tu, Rhys N Colson, Tuhin S. Maity, Robert X Lao, Katherine McJunkin, Michelle A Francisco |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Small RNA QH301-705.5 Science TRIM-NHL RNA-binding protein General Biochemistry Genetics and Molecular Biology Germline 03 medical and health sciences RNA interference small RNAs hemic and lymphatic diseases Gene expression Animals Gene Regulatory Networks Biology (General) Caenorhabditis elegans Caenorhabditis elegans Proteins Gene General Immunology and Microbiology biology 22G RNAs General Neuroscience RNA Genetics and Genomics General Medicine biology.organism_classification Chromosomes and Gene Expression Chromatin 3. Good health Cell biology 030104 developmental biology Germ Cells RNAi C. elegans Medicine RNA Interference Carrier Proteins Research Article |
| Zdroj: | eLife eLife, Vol 7 (2018) |
| ISSN: | 2050-084X |
| Popis: | Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma. |
| Databáze: | OpenAIRE |
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