Production of bioactive triterpenes by Eriobotrya japonica calli
| Autor: | Hoyoku Nishino, Harukuni Tokuda, Hideyuki Ito, Takashi Yoshida, Yoshie Takamatsu, Yoko Imayoshi, Susumu Shimura, Shoko Taniguchi, Hiroshi Sakagami, Daigo Sugita, Eri Kobayashi, Tsutomu Hatano |
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| Rok vydání: | 2002 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Magnetic Resonance Spectroscopy Skin Neoplasms Tormentic acid Antineoplastic Agents Plant Science Eriobotrya Horticulture Biology Pharmacognosy Biochemistry Mice chemistry.chemical_compound Triterpene Ursolic acid Maslinic acid Culture Techniques Tumor Cells Cultured Animals Humans Antigens Viral Rosaceae Molecular Biology Oleanolic acid Chromatography High Pressure Liquid chemistry.chemical_classification food and beverages General Medicine Salivary Gland Neoplasms biology.organism_classification Triterpenes Cell Transformation Neoplastic chemistry Callus Carcinoma Squamous Cell Mouth Neoplasms Cell Division |
| Zdroj: | Phytochemistry. 59:315-323 |
| ISSN: | 0031-9422 |
| DOI: | 10.1016/s0031-9422(01)00455-1 |
| Popis: | Callus tissue cultures induced from an axenic leaf of Eriobotrya japonica (Rosaceae) produced triterpenes in large amounts (ca. 50 mg/g dry wt). Nine triterpenes were characterized as ursolic acid, oleanolic acid, 2alpha-hydoxyursolic acid, maslinic acid, tormentic acid, 2alpha, 19alpha-dihydroxy-3-oxo-urs-12-en-28-oic acid, hyptadienic acid and a mixture of 3-O-cis-p-coumaroyltormentic acid and 3-O-trans-p-coumaroyltormentic acid. The triterpene composition in the callus tissues was noticeably different from that in intact leaves. The contents of tormentic acid with antidiabetic action, and 2alpha, 19alpha-dihydroxy-3-oxo-urs-12-en-28-oic acid with anti-HIV activity, were much larger than those in the intact leaves. All of the triterpenes isolated from the callus tissues showed an inhibitory effect comparable to (-)-epigallocatechin gallate (EGCG) of green tea on the activation of Epstein-Barr virus early antigen (EBV-EA) induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). 2alpha, 19alpha-Dihydroxy-3-oxo-urs-12-en-28-oic acid was the most potent inhibitor among them and caused a significant delay of two-stage carcinogenesis on mouse skin. |
| Databáze: | OpenAIRE |
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