Performance of two different Limulus amebocyte lysate assays for the quantitation of fungal glucan
| Autor: | Hafsa Cherid, J. David Miller, Mark Foto |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
Public Health Environmental and Occupational Health Fungi Pullulan macromolecular substances Curdlan Yeast Microbiology carbohydrates (lipids) stomatognathic diseases chemistry.chemical_compound Laminarin Biochemistry chemistry Species Specificity Limulus amebocyte lysate Paramylon Glucans Limulus Test Glucan Mannan Environmental Monitoring |
| Zdroj: | Journal of occupational and environmental hygiene. 8(9) |
| ISSN: | 1545-9632 |
| Popis: | This study examined the response of various forms and sources of glucans toward two different Limulus amebocyte lysate (LAL) methods, the modified LAL, and Glucatell. The glucans studied were curdlan, laminarin, yeast glucan, barley glucan, paramylon, pullulan, pustulan, mannan, and pachyman (as part of the Glucatell kit). Both methods provided largely similar results for each of the glucans; however, the Glucatell method yielded slightly higher responses to certain structures that may not necessarily be of fungal origin, leading to falsely greater positive results. The performance of each method to measure fungal glucan concentration specifically was then assessed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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