Heparin inhibits histamine release from canine mast cells
| Autor: | N. Inase, Stephen C. Lazarus, R. E. Schreck |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Pulmonary and Respiratory Medicine
Physiology Transplantation Heterologous Ionophore chemistry.chemical_element Mast-Cell Sarcoma Mice Nude Canine Mastocytoma Pharmacology Calcium Histamine Release Exocytosis chemistry.chemical_compound Mice Dogs Physiology (medical) medicine Tumor Cells Cultured Animals p-Methoxy-N-methylphenethylamine Mast Cells Calcimycin Heparin Dextran Sulfate Cell Biology Mast cell Kinetics medicine.anatomical_structure Biochemistry chemistry Liberation Histamine Neoplasm Transplantation medicine.drug |
| Zdroj: | The American journal of physiology. 264(4 Pt 1) |
| ISSN: | 0002-9513 |
| Popis: | To determine the role of heparin in mast cell exocytosis, we studied the effect of heparin on histamine release induced by compound 48/80 or calcium ionophore A23187 in canine mastocytoma cells (BR). Heparin caused concentration-dependent inhibition of compound 48/80-induced histamine release from mast cells (n = 4; P < 0.05) with a mean inhibitory concentration of 0.14 +/- 0.01 U/ml (mean +/- SE). Mean maximal inhibition was 69.3 +/- 2.0%. In contrast, heparin had no effect on calcium ionophore A23187-induced histamine release. Although benzyl alcohol, a preservative of pharmaceutical heparin, had no effect, purified heparin produced a similar inhibitory effect on compound 48/80-induced histamine release (n = 4; P < 0.05). The inhibitory effect of heparin on histamine release was rapid and was eliminated by washing cells. Dextran sulfate, a polysaccharide with negative charge density, produced a similar inhibitory effect on compound 48/80-induced histamine release (n = 4; P < 0.05). We conclude that heparin inhibits compound 48/80-induced exocytosis in mast cells probably by its negative charge density. |
| Databáze: | OpenAIRE |
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